Genomic and biochemical insights into the specificity of ETS transcription factors.
Genomic and biochemical insights into the specificity of ETS transcription factors.
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基因组和生化洞察ETS转录因子的特异性。
DOI：
10.1146/annurev.biochem.79.081507.103945
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发表时间：
2011
影响因子：
16.6
通讯作者：
Graves BJ
中科院分区：
文献类型：
--
作者：
Hollenhorst PC;McIntosh LP;Graves BJ
ETS蛋白是一组进化相关的DNA结合转录因子。这些蛋白质通过与特异性启动子和增强子结合并促进转录机制的其他组分的组装来指导不同正常和疾病状态下的基因表达。高度保守的DNA结合ETS结构域定义了该家族，并负责特异性识别共同的序列基序5′-GGA（A/T）-3′。因此，在这样的家族中获得生物调节的特异性是一个难题。我们目前的知识路线的功能多样性和DNA结合特异性，包括保守的ETS和PNT结构域的不同属性，参与侧翼结构化和非结构化的区域附加到这些动态域，翻译后修饰，和蛋白质伙伴关系与其他DNA结合蛋白和辅助调节。该综述强调了生物化学和生物物理方法的最新进展，以及从基因组研究中检测活细胞中ETS因子占用的见解。
ETS proteins are a group of evolutionarily related, DNA-binding transcriptional factors. These proteins direct gene expression in diverse normal and disease states by binding to specific promoters and enhancers and facilitating assembly of other components of the transcriptional machinery. The highly conserved DNA-binding ETS domain defines the family and is responsible for specific recognition of a common sequence motif, 5′-GGA(A/T)-3′. Attaining specificity for biological regulation in such a family is thus a conundrum. We present the current knowledge of routes to functional diversity and DNA binding specificity, including divergent properties of the conserved ETS and PNT domains, the involvement of flanking structured and unstructured regions appended to these dynamic domains, posttranslational modifications, and protein partnerships with other DNA-binding proteins and coregulators. The review emphasizes recent advances from biochemical and biophysical approaches, as well as insights from genomic studies that detect ETS-factor occupancy in living cells.