Identification of a critical region in the Drosophila ryanodine receptor that confers sensitivity to diamide insecticides
Identification of a critical region in the Drosophila ryanodine receptor that confers sensitivity to diamide insecticides
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DOI：
10.1016/j.ibmb.2013.06.006
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发表时间：
2013-09-01
影响因子：
3.8
通讯作者：
Cordova, Daniel
中科院分区：
文献类型：
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作者：
Tao, Yong;Gutteridge, Steven;Cordova, Daniel
邻氨基苯甲酰胺，其中包括新的商业杀虫剂，氯虫苯甲酰胺，是一个令人兴奋的新一类化学目标昆虫兰尼碱受体。这些受体调节储存的细胞内钙的释放，并在肌肉收缩中发挥关键作用。与昆虫一样，线虫表达兰尼丁受体，并且对植物生物碱兰尼丁敏感。然而，植物寄生线虫南方根结线虫对邻氨基苯甲酰胺不敏感。全长果蝇ryanodine受体在昆虫细胞系中的表达赋予对受体试剂咖啡因和ryanodine沿着对邻氨基苯二酰胺的纳摩尔敏感性。用来自根结线虫的果蝇C-末端的高度发散区域中的46个氨基酸片段替换导致对二酰胺杀虫剂缺乏敏感性的功能性RyR。这些发现表明，该区域是至关重要的昆虫兰尼碱受体的二酰胺敏感性。此外，这一区域可能有助于我们了解的差异选择性二酰胺表现出昆虫哺乳动物兰尼碱受体。(c)2013爱思唯尔有限公司保留所有权利。
Anthranilic diamides, which include the new commercial insecticide, chlorantraniliprole, are an exciting new class of chemistry that target insect ryanodine receptors. These receptors regulate release of stored intracellular calcium and play a critical role in muscle contraction. As with insects, nematodes express iyanodine receptors and are sensitive to the plant alkaloid, ryanodine. However the plant parasitic nematode, Meloidogyne incognita, is insensitive to anthranilic diamides. Expression of a full-length Drosophila melanogaster ryanodine receptor in an insect cell line confers sensitivity to the receptor agents, caffeine and ryanodine along with nanomolar sensitivity to anthranilic diamides. Replacement of a 46 amino acid segment in a highly divergent region of the Drosophila C-terminus with that from Meloidogyne results in a functional RyR which lack sensitivity to diamide insecticides. These findings indicate that this region is critical to diamide sensitivity in insect ryanodine receptors. Furthermore, this region may contribute to our understanding of the differential selectivity diamides exhibit for insect over mammalian ryanodine receptors. (c) 2013 Elsevier Ltd. All rights reserved.