Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae
Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae
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DOI：
10.1073/pnas.182380799
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发表时间：
2002-09-17
影响因子：
11.1
通讯作者：
Fraser, CM
中科院分区：
文献类型：
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作者：
Tettelin, H;Masignani, V;Fraser, CM
无乳链球菌是美国和欧洲新生儿细菌性败血症、肺炎和脑膜炎的主要原因，其 2,160,267 bp 基因组序列预计编码 2,175 个基因。无乳链球菌、肺炎链球菌、化脓性链球菌和其他完全测序的基因组之间的基因组比较确定了链球菌和无乳链球菌的特异性基因。这些计算机分析，结合使用全基因组微阵列在已测序的血清型 V 菌株 2603 V/R 和来自多个血清型的 19 个无乳链球菌菌株之间进行的比较基因组杂交实验，揭示了无乳链球菌菌株之间的遗传异质性，甚至是相同血清型的，并提供了对毒力机制进化的见解。
The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes. Genome comparisons among S. agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and the other completely sequenced genomes identified genes specific to the streptococci and to S. agalactiae. These in silico analyses, combined with comparative genome hybridization experiments between the sequenced serotype V strain 2603 V/R and 19 S. agalactiae strains from several serotypes using whole-genome microarrays, revealed the genetic heterogeneity among S. agalactiae strains, even of the same serotype, and provided insights into the evolution of virulence mechanisms.