Characterization of a xenograft model of human ovarian carcinoma which produces ascites and intraabdominal carcinomatosis in mice.
Characterization of a xenograft model of human ovarian carcinoma which produces ascites and intraabdominal carcinomatosis in mice.
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发表时间：
1984-11
期刊：
影响因子：
11.2
通讯作者：
T. C. Hamilton;R. Young;K. G. Louie;B. Behrens;W. Mckoy;K. Grotzinger;R. Ozols
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文献类型：
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作者：
T. C. Hamilton;R. Young;K. G. Louie;B. Behrens;W. Mckoy;K. Grotzinger;R. Ozols
我们使用体内和体外程序从人卵巢癌细胞系 NIH:OVCAR-3 中选择了一个具有腹膜内生长能力的细胞亚群。在雌性裸无胸腺小鼠中。 ip 之后注射这些细胞后，动物会出现类似于临床卵巢癌的转移扩散。疾病进展的特点是出现大量腹水、广泛的侵袭性腹水。肿瘤和肺转移。恶性腹水细胞是可移植的，表现出细胞质雄激素和雌激素受体，并表达卵巢癌相关抗原CA125（116,000单位/ml腹水上清液）。这些细胞还具有与原始细胞系 NIH:OVCAR-3 中存在的相同染色体标记。腹腔注射后的存活率腹水的传代取决于肿瘤细胞接种，范围从 4000 万个细胞的中位生存期为 39 天到 1150 万个移植细胞的中位生存期为 84 天。这种独特的体内模型的特点使其非常适合评估卵巢癌的新药和新颖的实验疗法。此外，这种体内模型与卵巢癌细胞系一起，可能对于研究特异性增加抗癌药物在肿瘤细胞中的细胞毒性而不增加正常组织中的毒性的药理学方法特别有用。激素受体的存在应有助于卵巢癌激素治疗的实验评估。
We have used in vivo and in vitro procedures to select a subpopulation of cells from the human ovarian carcinoma cell line, NIH:OVCAR-3, with the capacity to grow i.p. in female nude athymic mice. After i.p. injection of these cells, animals develop metastatic spread similar to that of clinical ovarian cancer. Disease progression is characterized by the development of massive ascites, extensive invasive i.p. tumors, and pulmonary metastases. The malignant ascites cells are transplantable, manifest cytoplasmic androgen and estrogen receptors, and express the ovarian cancer associated antigen CA125 (116,000 units/ml of ascites supernatant). The cells also have the same chromosome markers which were present in the original cell line, NIH:OVCAR-3. Survival following i.p. passage of ascites is dependent on tumor cell inoculum ranging from a median survival of 39 days with 40 million cells to 84 days for 11.5 million transplanted cells. The characteristics of this unique in vivo model make it well suited for the evaluation of new drugs and novel experimental therapies in ovarian cancer. In addition, this in vivo model, together with ovarian cancer cell lines, may prove particularly useful for the study of pharmacological ways to specifically increase the cytotoxicity of anticancer agents in tumor cells while not increasing toxicity in normal tissues. The presence of hormone receptors should facilitate the experimental evaluation of hormonal therapy in ovarian cancer.