Plasma microRNAs as novel biomarkers for early detection of lung cancer.
Plasma microRNAs as novel biomarkers for early detection of lung cancer.
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DOI：
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发表时间：
2011-08
影响因子：
1.4
通讯作者：
D. Zheng;S. Haddadin;Yong Wang;L. Gu;M. Perry;C. Freter;Michael X. Wang
中科院分区：
文献类型：
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作者：
D. Zheng;S. Haddadin;Yong Wang;L. Gu;M. Perry;C. Freter;Michael X. Wang
肺癌的早期诊断对于提高患者的生存率至关重要。microRNA（miRNAs）是一个由19- 25个核苷酸组成的非编码小RNA家族，在肺癌中经常发生失调。本研究的目的是研究循环miRNA用于肺癌早期检测的潜力。我们检索了已发表的文献中原发性肺癌的miRNA微阵列数据，并选择了15个在肺癌组织中最常上调的miRNA。分离包括miRNA的总血浆RNA，多聚腺苷酸化并逆转录成cDNA。通过实时RT-PCR测定74例肺癌患者和68例年龄匹配的无癌对照的miRNA水平。我们发现，与对照组相比，肺癌患者（包括I期患者）血浆中miR-155、miR-197和miR-182的水平显著升高（P<0.001）。这3种miRNA的组合在区分肺癌患者与对照组方面具有81.33%的灵敏度和86.76%的特异性。肺癌转移患者血浆中miR-155和miR-197水平高于无转移患者（P<0.05），化疗有效患者血浆中miR-155和miR-197水平低于无转移患者（P<0.001）。这些结果表明，miR-155、miR-197和miR-182可以作为肺癌早期检测的潜在非侵入性生物标志物。
A diagnosis of lung cancer at its early stages is vital for improving the survival rate of patients. MicroRNAs (miRNAs), a family of 19- to 25-nucleotide non-coding small RNAs, are frequently dysregulated in lung cancer. The objective of this study was to investigate the potential of circulating miRNAs for early detection of lung cancer. We searched the published literature for the miRNA microarray data of primary lung cancer and selected 15 miRNAs that were most frequently up-regulated in lung cancer tissues. Total plasma RNA including miRNAs was isolated, polyadenylated and reverse-transcribed into cDNAs. The levels of miRNAs were determined by real-time RT-PCR in 74 lung cancer patients and 68 age-matched cancer-free controls. We found that the levels of miR-155, miR-197, and miR-182 in the plasma of lung cancer including stage I patients were significantly elevated compared with controls (P<0.001). The combination of these 3 miRNAs yielded 81.33% sensitivity and 86.76% specificity in discriminating lung cancer patients from controls. The levels of miR-155 and miR-197 were higher in the plasma from lung cancer patients with metastasis than in those without metastasis (P<0.05) and were significantly decreased in responsive patients during chemotherapy (P<0.001). These results indicate that miR-155, miR-197, and miR-182 can be potential non-invasive biomarkers for early detection of lung cancer.