Diagnostic value of serum haptoglobin protein as hepatocellular carcinoma candidate marker complementary to α fetoprotein.
Diagnostic value of serum haptoglobin protein as hepatocellular carcinoma candidate marker complementary to α fetoprotein.
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DOI：
10.3892/or_00000982
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发表时间：
2010-11
期刊：
影响因子：
4.2
通讯作者：
H. Shu;X. Kang;K. Guo;Shan Li;Mei Li;Lu Sun;L. Gan;Yin‐kun Liu;X. Qin
中科院分区：
文献类型：
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作者：
H. Shu;X. Kang;K. Guo;Shan Li;Mei Li;Lu Sun;L. Gan;Yin‐kun Liu;X. Qin
80%以上的肝细胞癌发生于以乙肝病毒为基础的肝硬变患者，提示肝硬变患者是肝细胞癌的主要危险人群。甲胎蛋白(α)对肝癌患者甲胎蛋白阴性和/或小肿瘤的检测敏感性较差。在肝硬变患者中筛查血清标志物和监测肝细胞癌，可以在治疗可能更成功的早期阶段发现肝细胞癌。采用比较蛋白质组学方法对27例肝细胞癌患者和10例乙肝后肝硬变患者的血清进行筛选。2DE和MALDI-TOF-MS分析鉴定出5种差异显著的蛋白质(HP、HP2、前结合珠蛋白、SP40和SAA1)。结合珠蛋白(Hp)在肝细胞癌中的表达明显高于肝细胞癌。免疫印迹分析和比浊法检测结果显示，肝癌患者血清Hp水平显著高于LC患者(p<；0.05)，这与双向凝胶电泳法的结果一致。此外，联合应用HP和AFP可显著提高诊断准确率(AUC=0.838)。此外，血清幽门螺杆菌对甲胎蛋白阴性的肝癌患者也有潜在的诊断价值(AUC=0.763)。提示血清Hp可作为α胎儿蛋白的候选标志物。
More than 80% of hepatocellular carcinoma (HCC) arises in HBV based liver cirrhotic patients, suggesting that patients with cirrhosis form the main risk group of HCC. α fetoprotein (AFP) has poor sensitivity for the detection of AFP-negative and/or small tumors in HCC patients. Screening serum markers, along with HCC surveillance in patients with cirrhosis, can lead to the detection of HCC at an earlier stage, when curative therapy is likely to be more successful. Sera from 27 patients with HCC and 10 patients with HBV based liver cirrhosis (LC) were screened by comparative proteome analysis. Five significantly differential proteins (HP, Hp2, preprohaptoglobin, SP40 and SAA1) were identified using 2DE followed by MALDI-TOF-MS analysis. Haptoglobin (HP) was identified and found to be overexpressed in HCC as compared with LC. The result from Western blot analysis and turbidimetry detection showed serum levels of HP in HCC patients were significantly (p<0.05) higher than those in LC patients, which was consistent with the result of 2-DE. In addition, combining HP and AFP greatly improved the diagnostic accuracy (AUC=0.838). Additionally, serum HP also showed potential diagnostic value (AUC=0.763) for AFP-negative HCC patients. Altogether, it suggested that serum HP as a candidate marker complementary to α fetoprotein.