Ultraviolet-B damages corneal endothelium.
Ultraviolet-B damages corneal endothelium.
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紫外线 B 会损伤角膜内皮。
DOI：
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发表时间：
1984
期刊：
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通讯作者：
Jany Se
中科院分区：
文献类型：
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作者：
Cullen Ap;Chou Br;Halliburton Mg;Jany Se
：由于在上皮中产生更严重的损伤，因此不可能直接在体内观察急性紫外线（UV）诱导的角膜内皮损伤。为了量化损伤和评估内皮恢复，使用间接方法。用紫外线B（290 ~ 320 nm）照射有色家兔的眼睛，紫外线B通过光栅单色仪和适当的滤光片与高压1000 W反射氙弧灯的输出隔离。所用辐射的峰值波长为305 nm，半峰带宽为18 nm。角膜厚度的变化测量与修改蔡司（上科亨）角膜厚度计被用来跟踪上皮和内皮功能的变化。单独的上皮损伤导致24小时内最大厚度增加13.5%，并在24小时内恢复。在没有前葡萄膜受累的情况下，角膜厚度增加更大，表明内皮损伤，并在2天达到最大值，7天内恢复。足以干扰角膜消肿的内皮损伤阈值为0.12 J X cm-2。
: Direct in vivo observation of acute ultraviolet (UV)-induced corneal endothelial damage is not possible due to the more severe damage produced in the epithelium. In order to quantify damage and evaluate endothelial recovery an indirect method was used. Eyes of pigmented rabbits were irradiated with UV-B (290 to 320 nm) isolated from the output of a high-pressure 1000 W reflectorized Xenon arc lamp by a grating monochromator and appropriate filters. The peak wavelength of the radiation used was 305 nm, with a 18 nm bandwidth at half-maximum. Corneal thickness variations measured with a modified Zeiss (Oberkochen) pachometer were used to follow alterations in epithelial and endothelial function. Epithelial damage alone resulted in a maximum thickness increase of 13.5% within 24 hr with recovery within a further 24 hr. Greater increases in corneal thickness, in the absence of anterior uveal involvement, were taken to indicate endothelial damage, and reached maximum at 2 days, with recovery occurring in 7 days. The threshold for endothelial damage sufficient to disturb corneal deturgescence was 0.12 J X cm-2.