Single Locus Maintains Large Variation of Sex Reversal in Half-Smooth Tongue Sole (Cynoglossus semilaevis).
Single Locus Maintains Large Variation of Sex Reversal in Half-Smooth Tongue Sole (Cynoglossus semilaevis).
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DOI：
10.1534/g3.116.036822
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发表时间：
2017-02-09
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
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作者：
Jiang L;Li H
性别决定是个体性别发育和群体性别比的基本生物学过程。然而，对于某些物种来说，在发育过程中，初级性别可能会改变，个体可以发育成异性。性逆转可能发生在昆虫、爬行动物、两栖动物和鱼类中。在半滑舌鳎（Cynoglossus semilaevis），一些遗传雌性鱼不可逆地逆转为假单胞菌，导致养殖成本较高，由于在2年的生长期内，雄性鱼的生长速度较低。本研究通过高密度单核苷酸多态性（SNP）的全基因组关联研究，在半滑舌鳎中发现了一个对性逆转有较大控制作用的位点。该SNP位于Z染色体上的F-box和富含亮氨酸的重复蛋白17（FBXL 17）基因的第三内含子，并且它具有两个等位基因A和T。具有ZAW基因型的遗传雌性永远不会逆转为表型雄性，但具有ZTW基因型的遗传雌性有时会发生性逆转。该SNP解释了82.7%的遗传变异，或58.4%的表型变异。根据本研究结果，可以制定繁殖管理方案，以提高水产养殖中的表型雌性比例，并阐明半滑舌鳎性逆转的机制。我们预计，这些发现将对许多发生性逆转的收获物种的种群管理产生重大影响。
Sex determination is a fundamental biological process for individual sex development and population sex ratios. However, for some species, the primary sex might be altered during development, and individuals can develop into the opposite sex. Sex reversal may happen in insects, reptiles, amphibians, and fishes. In half-smooth tongue sole (Cynoglossus semilaevis), some genetically female fish irreversibly reverse to pseudomales, resulting in higher costs in aquaculture owing to a lower growth rate of male fish during a 2-yr growth period. Here, we identified a locus with large controlling effect on sex reversal in the half-smooth tongue sole through genome-wide association study with high-density single nucleotide polymorphisms (SNPs). This SNP is located at the third intron of the F-box and leucine rich repeat protein 17 (FBXL17) gene on the Z chromosome, and it has two alleles, A and T. Genetic females with ZAW genotypes will never reverse into phenotypic males, but those with ZTW genotypes can sometimes undergo sex reversal. This SNP explains 82.7% of the genetic variation, or 58.4% of the phenotypic variation. Based on our results, a reproductive management program could be developed to improve the phenotypic female ratio in aquaculture, and elucidate the mechanism of sex reversal in half-smooth tongue sole. We expect that these findings will have a substantial impact on the population management in many harvested species where sex reversal occurs.