The expression of calcitonin gene-related peptide in dorsal horn neurons of the mouse lumbar spinal cord
The expression of calcitonin gene-related peptide in dorsal horn neurons of the mouse lumbar spinal cord
复制标题
降钙素基因相关肽在小鼠腰脊髓背角神经元中的表达
作者：
T. Shi;Zhang Xu;T. Hökfelt
应用免疫组织化学和原位杂交技术，研究了正常状态下、单侧脊神经根切断后和局部秋水仙碱处理后小鼠脊髓降钙素基因相关肽（CGRP）的表达。在正常情况下，CGRP免疫反应（IR）纤维的密集丛中观察到的背角浅层与较低数量的纤维在深层。切断一侧脊神经根后7天，同侧脊神经浅层CGRP阳性神经纤维明显减少，在同侧第Ⅲ层可见明显的CGRP阳性神经元。CGRP mRNA阳性神经元在同侧和对侧背角的第III层观察。秋水仙碱处理并不明显增加CGRP-IR神经元的数量。结果表明，CGRP在小鼠背角局部神经元合成，这些神经元可能参与感觉加工。
Using immunohistochemistry and in situ hybridization the expression of calcitonin gene-related peptide (CGRP) was studied in the mouse spinal cord under normal conditions and after unilateral rhizotomy and after local colchicine treatment. Under normal conditions a dense plexus of CGRP-immunoreactive (IR) fibres was observed in the superfical layers of dorsal horns with lower numbers of fibers in deeper laminae. Seven days after unilateral rhizotomy, there was a marked reduction of CGRP-IR fibres in the ipsilateral superfical layers and distinctly CGRP-IR neurons could be detected in the ipsilateral lamina III. CGRP mRNA-positive neurons were observed in lamina III in both the ipsilateral and contralateral dorsal horn. Colchicine treatment did not markedly increase the number of CGRP-IR neurons. The results suggest that CGRP is synthesized in local dorsal horn neurons of the mouse, and these neurons presumably participate in sensory processing.