Monoclonal antibodies to circumsporozoite proteins identify the species of malaria parasite in infected mosquitoes
Monoclonal antibodies to circumsporozoite proteins identify the species of malaria parasite in infected mosquitoes
复制标题
环子孢子蛋白的单克隆抗体可识别受感染蚊子中的疟原虫种类
作者：
Fidel Zavala;Fidel Zavala;R. Gwadz;R. Gwadz;F. H. Collins;F. H. Collins;R. Nussenzweig;R. Nussenzweig;Victor Nussenzweig;Victor Nussenzweig
要了解疟疾的流行病学，就必须准确鉴定携带传染性子孢子的蚊媒种群。这通常是通过对个体蚊子进行费力的解剖和检查来确定的，通常是在每1,000只蚊子中含有子孢子的蚊子少于1只的地区。此外，它是不可能的，以确定物种的疟疾寄生虫在无脊椎动物宿主的形态标准。在这里，我们描述了两个敏感的免疫放射分析，使用单克隆抗体检测环子孢子（CS）蛋白抗原。这些测试可以检测，识别和量化子孢子在个别或池的蚊子;需要最小的努力，测试可以进行蚊子收集后很长一段时间从流行地区。
An understanding of the epidemiology of malaria depends on the accurate identification of the portion of the mosquito vector population carrying infectious sporozoites. This is usually determined by laborious dissection and examination of individual mosquitoes, often in areas where less than 1 per 1,000 mosquitoes contain sporozoites1. Moreover, it is impossible to identify the species of malaria parasite in the invertebrate host by morphological criteria. Here we describe two sensitive immunoradiometric assays which use monoclonal antibodies to detect circumsporozoite (CS) protein antigens. These tests can detect, identify and quantify sporozoites in individual or pools of mosquitoes; minimal effort is required and testing can be performed long after mosquito collection at sites removed from endemic areas.