Microbiopsy engineered for minimally invasive and suture-free sub-millimetre skin sampling.
Microbiopsy engineered for minimally invasive and suture-free sub-millimetre skin sampling.
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DOI：
10.12688/f1000research.2-120.v1
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Soyer, H Peter
中科院分区：
文献类型：
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作者：
Lin, Lynlee L;Prow, Tarl W;Soyer, H Peter
我们描述了一种亚毫米皮肤穿刺活检装置的发展，用于无痛和免缝合的皮肤取样，用于分子诊断和研究。传统的皮肤穿刺活检直径范围为2-4 mm。需要局部麻醉，通常使用缝线闭合伤口。我们的显微活检是0.50毫米宽和0.20毫米厚。显微活检装置由三个堆叠的医用级不锈钢板制成，逐渐变细到一个点，并在中心板内包含一个腔室以收集皮肤样本。我们观察到，使用反射共聚焦显微镜，该装置的应用在志愿者皮肤中产生0.21 ± 0.04 mm宽的穿刺部位。显微活检皮肤的组织切片显示0.22 ± 0.12 mm宽和0.26 ± 0.09 mm深的穿刺部位。对微活检志愿者的纵向观察显示，伤口在1天内闭合，7天后不可见。来自这些相同部位的反射共聚焦显微镜图像显示形成了一个微小的外壳，该外壳在3周内消退，并且肉眼完全无法检测到。在志愿者研究中，使用采样的DNA质量作为主要终点，对该装置的设计参数进行了优化，以进行分子分析。最后，对总RNA进行表征。优化后的装置可提取5.9 ± 3.4 ng DNA和9.0 ± 10.1 ng RNA。我们预见，微创分子采样将在诊断皮肤病学和皮肤研究中发挥越来越重要的作用。
We describe the development of a sub-millimetre skin punch biopsy device for painless and suture-free skin sampling for molecular diagnosis and research. Conventional skin punch biopsies range from 2-4 mm in diameter. Local anaesthesia is required and sutures are usually used to close the wound. Our microbiopsy is 0.50 mm wide and 0.20 mm thick. The microbiopsy device is fabricated from three stacked medical grade stainless steel plates tapered to a point and contains a chamber within the centre plate to collect the skin sample. We observed that the application of this device resulted in a 0.21 ± 0.04 mm wide puncture site in volunteer skin using reflectance confocal microscopy. Histological sections from microbiopsied skin revealed 0.22 ± 0.12 mm wide and 0.26 ± 0.09 mm deep puncture sites. Longitudinal observation in microbiopsied volunteers showed that the wound closed within 1 day and was not visible after 7 days. Reflectance confocal microscope images from these same sites showed the formation of a tiny crust that resolved by 3 weeks and was completely undetectable by the naked eye. The design parameters of the device were optimised for molecular analysis using sampled DNA mass as the primary end point in volunteer studies. Finally, total RNA was characterized. The optimised device extracted 5.9 ± 3.4 ng DNA and 9.0 ± 10.1 ng RNA. We foresee that minimally invasive molecular sampling will play an increasingly significant role in diagnostic dermatology and skin research.