Sensilla-Specific Expression of Odorant Receptors in the Desert Locust Schistocerca gregaria
Sensilla-Specific Expression of Odorant Receptors in the Desert Locust Schistocerca gregaria
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DOI：
10.3389/fphys.2019.01052
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发表时间：
2019-08-22
影响因子：
4
通讯作者：
Pregitzer, Pablo
中科院分区：
文献类型：
--
作者：
Jiang, Xingcong;Breer, Heinz;Pregitzer, Pablo
沙漠蝗虫Schistocerca gregaria识别多种化学信号，这些信号由位于形态可识别感器中的嗅觉感觉神经元接收。不同类型的感觉器含有具有不同生理特异性的嗅觉感觉神经元，即，它们对不同种类的化学信号作出反应。这些细胞的感受器特异性反应的分子基础是未知的，但可能是基于内源性受体库。为了探讨这个问题，试图阐明不同的气味受体（OR）是否可以在一个特定的感觉器的方式表达。分析超过80个OR类型的感器特异性表达的相关发现，绝大多数被发现在basiconica感器中表达，而只有三个OR类型的感器中表达。在一个感器单元内，即使在锥形感器的多细胞组装体中，许多OR类型仅在单个细胞中表达，然而，发现少数OR类型在通常排列在2-4个细胞的簇中的细胞聚生体中表达。通过比较不同若虫期的表达模式，证实了OR特异性细胞簇在发育过程中相继形成的观点。本研究结果揭示了蝗虫嗅觉系统的一些新颖而独特的特征，这将有助于揭示蝗虫嗅觉的复杂性。
The desert locust Schistocerca gregaria recognizes multiple chemical cues, which are received by olfactory sensory neurons housed in morphologically identifiable sensilla. The different sensillum types contain olfactory sensory neurons with different physiological specificities, i.e., they respond to different categories of chemical signals. The molecular basis for the sensilla-specific responsiveness of these cells is unknown, but probably based on the endogenous receptor repertoire. To explore this issue, attempts were made to elucidate whether distinct odorant receptors (ORs) may be expressed in a sensilla-specific manner. Analyzing more than 80 OR types concerning for a sensilla-specific expression revealed that the vast majority was found to be expressed in sensilla basiconica; whereas only three OR types were expressed in sensilla trichodea. Within a sensillum unit, even in the multicellular assembly of sensilla basiconica, many of the OR types were expressed in only a single cell, however, a few OR types were found to be expressed in a consortium of cells typically arranged in a cluster of 2-4 cells. The notion that the OR-specific cell clusters are successively formed in the course of development was confirmed by comparing the expression patterns in different nymph stages. The results of this study uncover some novel and unique features of locust olfactory system, which will contribute to unravel the complexity of locust olfaction.