Transcriptional landscape of trans-kingdom communication between Candida albicans and Streptococcus gordonii.
Transcriptional landscape of trans-kingdom communication between Candida albicans and Streptococcus gordonii.
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DOI：
10.1111/omi.12111
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发表时间：
2016-04
影响因子：
3.7
通讯作者：
Ramsdale M
中科院分区：
文献类型：
--
作者：
Dutton LC;Paszkiewicz KH;Silverman RJ;Splatt PR;Shaw S;Nobbs AH;Lamont RJ;Jenkinson HF;Ramsdale M
最近的研究表明，多形性真菌白色念珠菌的转录景观高度依赖于生长条件。在这里，我们使用双 RNA 测序方法鉴定了 299 个白色念珠菌和 72 个戈登链球菌基因，这些基因由于共培养这些人类口腔微生物而特异性上调或下调。白色念珠菌中涉及化学刺激反应、调节、体内平衡、蛋白质修饰和细胞周期的 75 个基因统计上调（P ≤ 0.05），而主要涉及运输和翻译的 36 个基因下调。丝状相关 TEC1 和 FGR42 基因以及 ALS1 粘附素基因的上调与先前的证据一致，即戈登酵母促进了白色念珠菌酵母向菌丝的转变。白色念珠菌中精氨酸生物合成所需基因表达的增加可能表明一种新的氧化应激反应。 S. gordonii 对 C. albicans 的转录反应不太显着，只有 8 个 S. gordonii 基因显着 (P ≤ 0.05) 上调 ≥ 两倍（glpK、rplO、celB、rplN、rplB、rpsE、ciaR 和 gat）。表达模式表明，来自戈登沙门氏菌的信号在白色念珠菌中引起正丝状反应，而戈登沙门氏菌似乎在转录上受白色念珠菌的影响较小。
Recent studies have shown that the transcriptional landscape of the pleiomorphic fungus Candida albicans is highly dependent upon growth conditions. Here using a dual RNA-seq approach we identified 299 C. albicans and 72 Streptococcus gordonii genes that were either up- or down-regulated specifically as a result of co-culturing these human oral cavity microorganisms. Seventy five C. albicans genes involved in responses to chemical stimuli, regulation, homeostasis, protein modification and cell cycle were statistically (P ≤0.05) up-regulated, while 36 genes mainly involved in transport and translation were down-regulated. Up-regulation of filamentation-associated TEC1 and FGR42 genes, and of ALS1 adhesin gene, concurred with previous evidence that the C. albicans yeast to hypha transition is promoted by S. gordonii. Increased expression of genes required for arginine biosynthesis in C. albicans was potentially indicative of a novel oxidative stress response. The transcriptional response of S. gordonii to C. albicans was less dramatic, with only eight S. gordonii genes significantly (P ≤0.05) up-regulated ≥ twofold (glpK, rplO, celB, rplN, rplB, rpsE, ciaR, and gat). The expression patterns suggest that signals from S. gordonii cause a positive filamentation response in C. albicans, while S. gordonii appears to be transcriptionally less influenced by C. albicans.