Foliar water uptake as a source of hydrogen and oxygen in plant biomass.
Foliar water uptake as a source of hydrogen and oxygen in plant biomass.
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DOI：
10.1093/treephys/tpac055
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发表时间：
2022-11-08
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影响因子：
4
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中科院分区：
文献类型：
--
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世界各地的入门生物学课程通常教导植物通过其根吸收水分，但不幸的是，通过叶子吸收水分以及随后运输和利用这些水来形成生物质仍然是一个主要局限于专家的领域。最近的研究发现，叶片水分吸收是陆地植物的一个重要净水源。人们对开发一种新的模型越来越感兴趣，该模型既包括叶吸水(液体形式)，也包括根吸水，以解释叶水和树轮中的氢和氧同位素比率，需要一种区分这两种水源的方法。因此，在这项研究中，我设计了一种新的标记方法，利用两种不同的水源，一种是富氢水源(HDO+δD2O；‰，δD=70.00×104D2O；‰D=70.00×10 4δ18O=94.1‰)，另一种是富氧-18水源(H_218O；δD_0=−85‰，δ18O‰=91.1×10~4)，同时标记叶吸收水和根吸收水，并量化它们对植物生物量的相对贡献。利用这种新的方法，我在这里提出了证据，对于水分充足的柳杉来说，雨季进入新叶纤维素中的氢和氧主要来自叶吸收水(69%来自叶吸收水，31%来自根吸收水)，而新根纤维素主要来自根吸收水(20%来自叶吸收水，80%来自根吸收水)，新枝木质部介于两者之间(55%来自叶吸收水，45%来自根吸收水)。本研究首次采用的双标记方法实现了叶吸收水和根吸收水的分离和同时标记，为研究这些水在陆地植物中的吸收、运输和同化过程提供了一种新的工具。建议对光合作用图进行修改。之前：一张典型的光合作用图像显示，在植物生物量中，只有根的水分吸收作为氢气和氧气的来源。之后：建议的修改显示，叶片的水分吸收也是一个来源。这项研究首次量化了植物叶吸收水和根吸收水中氢和氧对植物生物量中氢和氧的相对贡献，并首次提供了明确的实验证据，表明生物体中相当大比例的氢和氧(如纤维素中的氢和氧)来自叶吸收水。
Introductory biology lessons around the world typically teach that plants absorb water through their roots, but, unfortunately, absorption of water through leaves and subsequent transport and use of this water for biomass formation remains a field limited mostly to specialists. Recent studies have identified foliar water uptake as a significant net water source for terrestrial plants. The growing interest in the development of a new model that includes both foliar water uptake (in liquid form) and root water uptake to explain hydrogen and oxygen isotope ratios in leaf water and tree rings demands a method for distinguishing between these two water sources. Therefore, in this study, I have devised a new labelling method that utilizes two different water sources, one enriched in deuterium (HDO + D2O; δD = 7.0 × 10 4‰, δ18O = 4.1‰) and one enriched in oxygen-18 (H218O; δD = −85‰, δ18O = 1.1 × 104‰), to simultaneously label both foliar-absorbed and root-absorbed water and quantify their relative contributions to plant biomass. Using this new method, I here present evidence that, in the case of well-watered Cryptomeria japonica D. Don, hydrogen and oxygen incorporated into new leaf cellulose in the rainy season derives mostly from foliar-absorbed water (69% from foliar-absorbed water and 31% from root-absorbed water), while that of new root cellulose derives mostly from root-absorbed water (20% from foliar-absorbed water and 80% from root-absorbed water), and new branch xylem is somewhere in between (55% from foliar-absorbed water and 45% from root-absorbed water). The dual-labelling method first implemented in this study enables separate and simultaneous labelling of foliar-absorbed and root-absorbed water and offers a new tool to study the uptake, transport and assimilation processes of these waters in terrestrial plants. Suggested modification to photosynthesis diagrams. Before: a typical photosynthesis image shows only root water uptake as a source of hydrogen and oxygen in plant biomass. After: suggested modification shows foliar water uptake also as a source. This is the first study that quantifies relative contributions of hydrogen and oxygen from foliar-absorbed water and root-absorbed water to hydrogen and oxygen in plant biomass, and offers the first clear experimental evidence that a significant proportion of hydrogen and oxygen in the biomass (such as hydrogen and oxygen in cellulose) originates from foliar-absorbed water.
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影响因子：
4.6
作者：
Cernusak LA;Ubierna N;Jenkins MW;Garrity SR;Rahn T;Powers HH;Hanson DT;Sevanto S;Wong SC;McDowell NG;Farquhar GD
通讯作者：
Farquhar GD
影响因子：
64.8
作者：
DAWSON, TE;EHLERINGER, JR
通讯作者：
EHLERINGER, JR
影响因子：
7.3
作者：
Barnard, Romain L.;Salmon, Yann;Buchmann, Nina
通讯作者：
Buchmann, Nina
影响因子：
56.9
作者：
DENIRO, MJ;EPSTEIN, S
通讯作者：
EPSTEIN, S
影响因子：
4.2
作者：
Begum, Shahanara;Nakaba, Satoshi;Funada, Ryo
通讯作者：
Funada, Ryo