Distribution, phylogenetic diversity and physiological characteristics of epsilon-Proteobacteria in a deep-sea hydrothermal field
Distribution, phylogenetic diversity and physiological characteristics of epsilon-Proteobacteria in a deep-sea hydrothermal field
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DOI：
10.1111/j.1462-2920.2005.00856.x
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发表时间：
2005-10-01
影响因子：
5.1
通讯作者：
Sako, Y
中科院分区：
文献类型：
--
作者：
Nakagawa, S;Takai, K;Sako, Y
epsilon -变形菌门越来越被认为是一种具有重要生态意义的细菌群，特别是在深海热液环境中。本研究采用培养依赖和非培养依赖两种方法，研究了冲绳海槽中部Iheya北油田深海热液喷口附近不同微生物栖息地中epsilon-Proteobacteria的空间分布、多样性和生理特征。研究的生境为热液柱内外、环节动物多毛类管。此外，我们在排气口附近部署了殖民装置。多毛类管中有生理上和系统发育上多样化的微生物群落。原位样品主要由epsilon-Proteobacteria定植。epsilon-Proteobacteria菌株的能量代谢具有高度的多样性。代谢性状生成的树拓扑与16S rRNA树的树拓扑存在显著差异（P = 0.000），表明目前基于16S rRNA基因的分析不能提供足够的信息来推断epsilon-Proteobacteria的生理特性。然而，epsilon-Proteobacteria在不同微生物栖息地的可培养性在系统发育亚群之间存在差异。硫单胞菌的成员具有很强的可培养性，而其他系统发育亚群似乎在海水中失去了可培养性，这可能是因为对氧气的敏感性。这些结果为深海热液喷口epsilon-Proteobacteria的生态生理特性提供了新的认识，这是以前从未通过16S rRNA基因的比较分析来评估的。
Epsilon-Proteobacteria is increasingly recognized as an ecologically significant group of bacteria, particularly in deep-sea hydrothermal environments. In this study, we studied the spatial distribution, diversity and physiological characteristics of the epsilon-Proteobacteria in various microbial habitats in the vicinity of a deep-sea hydrothermal vent occurring in the Iheya North field in the Mid-Okinawa Trough, by using culture-dependent and -independent approaches. The habitats studied were inside and outside hydrothermal plume, and annelid polychaete tubes. In addition, we deployed colonization devices near the vent emission. The polychaete tubes harboured physiologically and phylogenetically diverse microbial community. The in situ samplers were predominantly colonized by epsilon-Proteobacteria. Energy metabolism of epsilon-Proteobacteria isolates was highly versatile. Tree topology generated from the metabolic traits was significantly different (P = 0.000) from that of 16S rRNA tree, indicating current 16S rRNA gene-based analyses do not provide sufficient information to infer the physiological characteristics of epsilon-Proteobacteria. Nevertheless, culturability of epsilon-Proteobacteria in various microbial habitats differed among the phylogenetic subgroups. Members of Sulfurimonas were characterized by the robust culturability, and the other phylogenetic subgroups appeared to lose culturability in seawater, probably because of the sensitivity to oxygen. These results provide new insight into the ecophysiological characteristics of the deep-sea hydrothermal vent epsilon-Proteobacteria, which has never been assessed by comparative analysis of the 16S rRNA genes.