A Snapshot of the Emerging Tomato Genome Sequence
A Snapshot of the Emerging Tomato Genome Sequence
复制标题
新兴番茄基因组序列的快照
DOI：
10.3835/plantgenome2008.08.0005
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发表时间：
2009-05-01
期刊：
影响因子：
4.2
通讯作者：
Stiekema, Willem
中科院分区：
文献类型：
--
作者：
Mueller, Lukas A.;Lankhorst, Rene Klein;Stiekema, Willem
番茄（Solanum lycopersicum L.）的基因组作为“国际茄科基因组计划（SOL）：多样性和适应性的系统方法”倡议的一部分，一个由10个国家（韩国、中国、英国、印度、荷兰、法国、日本、西班牙、意大利和美国）组成的国际财团正在对该基因组进行测序。番茄基因组测序计划使用有序细菌人工染色体（BAC）方法来产生高质量的番茄常染色质基因组序列，用作茄科和真核生物的参考基因组。序列保存在GenBank和SOL基因组学网络（SGN）。目前，大约有1000个BAC已经完成或正在进行中，占基因组常染色质部分的三分之一以上。国际番茄注释组也在进行注释工作。常染色质中的预期基因数量近似于40，000个，这是基于对11%的最终序列的初步注释的估计。在这里，我们提出了这第一个快照的新兴番茄基因组和它的注释，一个简短的比较与马铃薯（马铃薯L。）序列数据，以及研究人员利用这一新资源的工具也被提出。将来，全基因组鸟枪技术将与BAC-by-BAC方法相结合，以覆盖整个番茄基因组。高质量的参考常染色质番茄序列预计将于2010年完成。
The genome of tomato (Solanum lycopersicum L.) is being sequenced by an international consortium of 10 countries (Korea, China, the United Kingdom, India, the Netherlands, France, Japan, Spain, Italy, and the United States) as part of the larger "International Solanaceae Genome Project (SOL): Systems Approach to Diversity and Adaptation" initiative. The tomato genome sequencing project uses an ordered bacterial artificial chromosome (BAC) approach to generate a high-quality tomato euchromatic genome sequence for use as a reference genome for the Solanaceae and euasterids. Sequence is deposited at GenBank and at the SOL Genomics Network (SGN). Currently, there are around 1000 BACs finished or in progress, representing more than a third of the projected euchromatic portion of the genome. An annotation effort is also underway by the International Tomato Annotation Group. The expected number of genes in the euchromatin is similar to 40,000, based on an estimate from a preliminary annotation of 11% of finished sequence. Here, we present this first snapshot of the emerging tomato genome and its annotation, a short comparison with potato (Solanum tuberosum L.) sequence data, and the tools available for the researchers to exploit this new resource are also presented. In the future, whole-genome shotgun techniques will be combined with the BAC-by-BAC approach to cover the entire tomato genome. The high-quality reference euchromatic tomato sequence is expected to be near completion by 2010.