ChIA-PET analysis of transcriptional chromatin interactions.
ChIA-PET analysis of transcriptional chromatin interactions.
复制标题
DOI：
10.1016/j.ymeth.2012.08.009
复制
发表时间：
2012-11
期刊：
影响因子：
4.8
通讯作者：
Jingyao Zhang;H. Poh;Su Qin Peh;Y. Sia;Guoliang Li;F. Mulawadi;Yufen Goh;M. Fullwood;W. Sung;Xiaoan Ruan;Y. Ruan
中科院分区：
文献类型：
--
作者：
Jingyao Zhang;H. Poh;Su Qin Peh;Y. Sia;Guoliang Li;F. Mulawadi;Yufen Goh;M. Fullwood;W. Sung;Xiaoan Ruan;Y. Ruan
特异性DNA调控元件之间的长距离染色质接触在基因表达调控中起着关键作用，并且在三维（3D）染色质结构中对这些相互作用的全局表征对于理解信号网络和细胞状态至关重要。使用配对末端标签测序的染色质相互作用分析（ChIA-PET）是一种将功能性染色质结构转化为数百万个短标签序列的方法。结合染色质免疫沉淀（ChIP），邻近连接和高通量测序，ChIA-PET提供了一个全球性的和公正的询问与特定蛋白质因子相关的高阶染色质结构。在这里，我们描述了详细的程序ChIA-PET方法，解开转录相关的染色质接触模型人类细胞系。
Long-range chromatin contacts between specific DNA regulatory elements play a pivotal role in gene expression regulation, and a global characterization of these interactions in the 3-dimensional (3D) chromatin structure is imperative in understanding signaling networks and cell states. Chromatin Interaction Analysis using Paired-End Tag sequencing (ChIA-PET) is a method which converts functional chromatin structure into millions of short tag sequences. Combining Chromatin Immunoprecipitation (ChIP), proximity ligation and high-throughput sequencing, ChIA-PET provides a global and unbiased interrogation of higher-order chromatin structures associated with specific protein factors. Here, we describe the detailed procedures of the ChIA-PET methodology, unraveling transcription-associated chromatin contacts in a model human cell line.