Plant lanosterol synthase: divergence of the sterol and triterpene biosynthetic pathways in eukaryotes.
Plant lanosterol synthase: divergence of the sterol and triterpene biosynthetic pathways in eukaryotes.
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DOI：
10.1093/pcp/pcj032
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发表时间：
2006-05
影响因子：
4.9
通讯作者：
Satoru Sawai;T. Akashi;N. Sakurai;Hideyuki Suzuki;D. Shibata;S. Ayabe;T. Aoki
中科院分区：
文献类型：
--
作者：
Satoru Sawai;T. Akashi;N. Sakurai;Hideyuki Suzuki;D. Shibata;S. Ayabe;T. Aoki
甾醇是真核生物的基本成分，通过环状三萜、羊毛甾醇（真菌和动物）或环阿屯醇（植物）生物合成。通过对羊毛甾醇合成酶（LAS）缺陷突变酵母的互补作用和积累的羊毛甾醇的结构鉴定，显示百脉根OSC7的cDNA编码LAS。一个双位点定向突变体的OSC7，其中的反应特异性的氨基酸残基的关键被改变为环阿屯醇合酶（CAS）的类型，产生parkeol和环阿屯醇。保守区的多个氨基酸序列比对表明，不同真核生物谱系的LAS出现从祖先CAS的趋同进化。
Sterols, essential eukaryotic constituents, are biosynthesized through either cyclic triterpenes, lanosterol (fungi and animals) or cycloartenol (plants). The cDNA for OSC7 of Lotus japonicus was shown to encode lanosterol synthase (LAS) by the complementation of a LAS-deficient mutant yeast and structural identification of the accumulated lanosterol. A double site-directed mutant of OSC7, in which amino acid residues crucial for the reaction specificity were changed to the cycloartenol synthase (CAS) type, produced parkeol and cycloartenol. The multiple amino acid sequence alignment of a conserved region suggests that the LAS of different eukaryotic lineages emerged from the ancestral CAS by convergent evolution.