Identification of dominant spoilage bacteria in sea cucumber protein peptide powders (SCPPs) and methods for controlling the growth of dominant spoilage bacteria by inhibiting hygroscopicity
Identification of dominant spoilage bacteria in sea cucumber protein peptide powders (SCPPs) and methods for controlling the growth of dominant spoilage bacteria by inhibiting hygroscopicity
复制标题
海参蛋白肽粉（SCPP）中优势腐败菌的鉴定及通过抑制吸湿性控制优势腐败菌生长的方法
DOI：
10.1016/j.lwt.2020.110355
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发表时间：
2021-01-01
影响因子：
6
通讯作者：
Lin, Songyi
中科院分区：
文献类型：
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作者：
Li, Xinran;Yang, Ruiwen;Lin, Songyi
以往的研究发现，高活性海参肽粉（SCPP）具有相对显著的吸湿特性。由于SCPP的吸湿性，观察到诸如液化、味道改变和活性降低的问题。因此，为了开发一种新的方法来准确，快速地分析和鉴定由于吸湿性SCPP而导致的优势腐败菌，我们将SCPP置于75%RH和25 ℃下，以监测在这种高吸湿性环境中储存90天的样品中的微生物属和种。贮藏90 d的SCPP的抗氧化活性显著低于天然SCPP（P < 0.05）。SCPP的吸湿性导致优势腐败菌的生长。在高湿环境中贮藏5d时，细菌占优势（45.89%）。在高湿环境中贮藏15 d时，细菌（41.35%）和γ-变形菌（40.96%）为优势菌，未鉴定的蓝藻（20.06%）急剧下降至0.04%（P < 0.05）。SCPPs在高吸湿环境中的劣变与厚壁菌门和变形菌门有关，主要包括芽孢杆菌属、嗜冷菌属和奇异菌属。
Previous studies have found that high active sea cucumber peptide powders (SCPPs) have relatively significant hygroscopic properties. Problems such as liquefaction, altered taste, and decreased activity are observed owing to the hygroscopic nature of SCPPs. Therefore, to develop a new method for accurate, rapid analysis and identification of dominant spoilage bacteria due to hygroscopic SCPPs, we placed SCPPs in 75% RH and 25 degrees C to monitor the microbial genera and species in samples stored for 90 days in this highly hygroscopic environment. The antioxidant activity of SCPPs stored for 90 days was significantly lower than that of the nature SCPPs (P < 0.05). The hygroscopic nature of SCPPs resulted in the growth of dominant spoilage bacteria. Bacilli (45.89%) were the dominant bacteria at 5 days of storage in a highly hygroscopic environment. Bacilli (41.35%) and Gammaproteobacteria (40.96%) were the dominant bacteria at 15 days of storage in a highly hygroscopic environment, and the number of unidentified Cyanobacteria (20.06%) was sharply reduced to 0.04% (P < 0.05). The deterioration of SCPPs in the highly hygroscopic storage environment was related to Firmicutes and Proteobacteria, which mainly include Bacillus, Psychrobacter and Atopobium.