Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena
Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena
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DOI：
10.1073/pnas.95.13.7480
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发表时间：
1998-06-23
影响因子：
11.1
通讯作者：
Allis, CD
中科院分区：
文献类型：
--
作者：
Wei, Y;Mizzen, CA;Allis, CD
H3磷酸化在哺乳动物细胞中与有丝分裂时间相关，在纤毛原生动物中与有丝分裂空间相关。例如，在体外生长的嗜热四膜虫细胞中，H3磷酸化可在有丝分裂的生殖细胞微核中检测到，但在无丝分裂的体细胞大核中检测不到。在这里，我们证明，微核H3磷酸化发生在一个单一的网站（Ser-10）在组蛋白H3的氨基末端结构域，在哺乳动物细胞有丝分裂过程中磷酸化相同的网站。使用对Ser-10磷酸化H3特异性的抗体，我们表明，在四膜虫中，这种修饰与微核的有丝分裂和减数分裂相关，其方式与染色体浓缩密切一致。我们的数据表明，在Ser-10的H3磷酸化是一个高度保守的真核生物之间的事件，并可能参与有丝分裂和减数分裂染色体凝聚。
H3 phosphorylation has been correlated with mitosis temporally in mammalian cells and spatially in ciliated protozoa, In logarithmically growing Tetrahymena thermophila cells, for example, H3 phosphorylation can be detected in germline micronuclei that divide mitotically but not in somatic macronuclei that divide amitotically. Here, we demonstrate that micronuclear H3 phosphorylation occurs at a single site (Ser-10) in the amino-terminal domain of histone H3, the same site phosphorylated during mitosis in mammalian cells. Using an antibody specific for Ser-10 phosphorylated H3, we show that, in Tetrahymena, this modification is correlated with mitotic and meiotic divisions of micronuclei in a fashion that closely coincides with chromosome condensation. Our data suggest that H3 phosphorylation at Ser-10 is a highly conserved event among eukaryotes and is likely involved in both mitotic and meiotic chromosome condensation.