Comparing guiding track requirements for myosin- and kinesin-powered molecular shuttles
Comparing guiding track requirements for myosin- and kinesin-powered molecular shuttles
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DOI：
10.1021/nl8010885
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发表时间：
2008-08-01
期刊：
影响因子：
10.8
通讯作者：
Hess, Henry
中科院分区：
文献类型：
--
作者：
Nitta, Takahiro;Tanahashi, Akihito;Hess, Henry
利用马达蛋白作为发动机的纳米级运输系统的设计进展迅速。在这里，比较了基于肌动蛋白/肌球蛋白和基于微管/驱动蛋白的分子穿梭机对轨道设计的要求。为此，肌动蛋白丝在肌球蛋白覆盖的表面上滑动的轨迹持续长度已被实验确定为等于8.8 +/- 2 μ m。这种测量补充了早期确定的轨迹持久性长度的微管上的驱动蛋白涂层的表面上滑行，并使驱动蛋白和肌球蛋白电机驱动系统的可访问的轨道设计的比较。尽管肌动蛋白丝的刚度比微管小200倍，但肌动蛋白丝的肌球蛋白轨迹的尺寸必须与微管的驱动蛋白轨迹的尺寸非常相似（半径大于200 nm，宽度小于0.9 μ m，而600 nm和19 μ m）。滑翔速度的差异，需要额外的考虑在轨道模块的设计。
The design of nanoscale transport systems utilizing motor proteins as engines has advanced rapidly. Here, actin/myosin- and microtubule/kinesin-based molecular shuttles are compared with respect to their requirements for track designs. To this end, the trajectory persistence length of actin filaments gliding on myosin-coated surfaces has been experimentally determined to be equal to 8.8 +/- 2 mu m. This measurement complements an earlier determination of the trajectory persistence length of microtubules gliding on kinesin-coated surfaces and enables a comparison of the accessible track designs for kinesin and myosin motor-powered systems. Despite the 200-fold smaller stiffness of actin filaments compared to that of microtubules, the dimensions of myosin tracks for actin filaments have to be quite similar to the dimensions of kinesin tracks for microtubules (radii larger than 200 nm and widths smaller than 0.9 mu m compared to 600 nm and 19 mu m). The difference in gliding speed is shown to require additional consideration in the design of track modules.