Synergistic Effect of Kaempferol Glycosides Purified from Laurus nobilis and Fluoroquinolones on Methicillin-Resistant Staphylococcus aureus
Synergistic Effect of Kaempferol Glycosides Purified from Laurus nobilis and Fluoroquinolones on Methicillin-Resistant Staphylococcus aureus
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DOI：
10.1248/bpb.32.489
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发表时间：
2009-03-01
影响因子：
2
通讯作者：
Tsuchiya, Tomofusa
中科院分区：
文献类型：
--
作者：
Liu, Mei-Hua;Otsuka, Nao;Tsuchiya, Tomofusa
在以前的研究中，我们报道了从樟树中分离得到的两个山奈酚糖苷：山奈酚-3-O-3-O-α-L-(2‘’，4‘-二-E-对香豆酰)-鼠李糖苷(C2)和山奈酚-3-O-α-L-(2’-E-对香豆酰-4‘’-Z-对香豆酰)-鼠李糖苷(C3)，它们对耐甲氧西林的金黄色葡萄球菌(MRSA)和耐万古霉素的肠球菌具较强的抗菌活性。此后，我们发现这些化合物大大降低了某些氟喹诺酮类药物在MRSA中的最低抑菌浓度(MIC)。换句话说，C2和C3大大增强了氟喹诺酮类药物的抗MRSA活性。C2和C3与氟喹诺酮类药物具有协同作用。亲水性氟喹诺酮类药物如诺氟沙星和环丙沙星对其有增强作用，而疏水性喹诺酮类药物则无此作用。我们还发现诺氟沙星降低了C2和C3的MIC值。其效果是协同作用的。并对可能的协同作用机理进行了探讨。
In a previous study, we reported that two kaempferol glycosides isolated from Laurus nobilis L., kaempferol-3-O- 3-O-alpha-L-(2 '',4 ''-di-E-p-couamaroyl)-rhamnoside (C2) and kaempferol-3-O-alpha-L-(2 ''-E-p-coumaroyl-4 ''-Z-p-coumaroyl)-rhamnoside (C3), showed strong antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci. Thereafter we found that these compounds greatly reduced the minimum inhibitory concentrations (MICs) of some fluoroquinolones in MRSA. In other words, C2 and C3 greatly potentiated anti-MRSA activity of fluoroquinolones. The effect of C2 and C3 with fluoroquinolones was found to be synergistic. The potentiation activity was observed with hydrophilic fluoroquinolones, such as norfloxacin and ciprofloxacin, but not with hydrophobic quinolones. We also found that norfloxacin reduced MICs of C2 and C3. The effect was synergistic. Possible mechanism of the synergistic effect was discussed.