Novel features of radiation-induced bystander signaling in Arabidopsis thaliana demonstrated using root micro-grafting
Novel features of radiation-induced bystander signaling in Arabidopsis thaliana demonstrated using root micro-grafting
复制标题
使用根微嫁接证明了拟南芥中辐射诱导的旁观者信号传导的新特征
DOI：
10.4161/psb.22451
复制
发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
L. Wu
中科院分区：
文献类型：
--
作者：
T. Wang;F. Li;W. Xu;P. Bian;Y. Wu;L. Wu
辐射诱导的旁观者效应(Ribe)已经在整个生物体、体外单细胞培养模型和体外多细胞组织模型中得到了很好的证实，但其潜在的机制尚不清楚，包括旁观者信号的时间和空间过程。体内的Ribe已被证明存在于模式植物拟南芥(A.thaliana)中。重要的是，这种独特的植物嫁接为在整个植物背景下研究旁观者信号的时间和空间过程提供了一种微妙的方法。在我们之前的研究中，植物中旁观者信号的时间过程已经通过根微嫁接技术得到了很好的证明。在这项研究中，我们进一步研究了多个旁观者信号的时间协作模式，旁观者信号的方向性，以及旁观者组织对旁观者信号的影响。结果表明，当非同步产生的旁观者信号在同一时间段到达旁观者组织时，也能有效地诱导旁观者反应，但当它们以相反的顺序进入旁观者组织时，则不能有效地诱导旁观者反应。倒根嫁接中没有旁观者反应，说明沿根的旁观者信号可能具有方向性。旁观者信号被证明是独立于旁观者组织的。
Radiation-induced bystander effects (RIBE) have been well demonstrated in whole organisms, as well as in single-cell culture models in vitro and multi-cellular tissues models in vitro, however, the underlying mechanisms remain unclear, including the temporal and spatial course of bystander signaling. The RIBE in vivo has been shown to exist in the model plant Arabidopsis thaliana (A. thaliana). Importantly, the unique plant grafting provides a delicate approach for studying the temporal and spatial course of bystander signaling in the context of whole plants. In our previous study, the time course of bystander signaling in plants has been well demonstrated using the root micro-grafting technique. In this study, we further investigated the temporal cooperation pattern of multiple bystander signals, the directionality of bystander signaling, and the effect of bystander tissues on the bystander signaling. The results showed that the bystander response could also be induced efficiently when the asynchronously generated bystander signals reached the bystander tissues in the same period, but not when they entered into the bystander tissues in an inversed sequence. The absence of bystander response in root-inversed grafting indicated that the bystander signaling along roots might be of directionality. The bystander signaling was shown to be independent of the bystander tissues.
登录
查看更多内容
DOI：
10.1073/pnas.182209699
发表时间：
2002-10
影响因子：
11.1
作者：
L. Xue;N. Butler;G. Makrigiorgos;S. Adelstein;A. Kassis
通讯作者：
L. Xue;N. Butler;G. Makrigiorgos;S. Adelstein;A. Kassis
影响因子：
6.9
作者：
Kehr, Julia;Buhtz, Anja
通讯作者：
Buhtz, Anja
DOI：
10.1016/j.mrfmmm.2005.08.012
发表时间：
2006-05-11
影响因子：
2.3
作者：
Belyakov, Oleg V.;Folkard, Melvyn;Michael, Barry D.
通讯作者：
Michael, Barry D.
DOI：
10.1073/pnas.0505020102
发表时间：
2005-10-04
影响因子：
11.1
作者：
Belyakov, OV;Mitchell, SA;Brenner, DJ
通讯作者：
Brenner, DJ
影响因子：
4.6
作者：
Hei, TK
通讯作者：
Hei, TK