The Detection of Glycosphingolipids in Brain Tissue Sections by Imaging Mass Spectrometry Using Gold Nanoparticles
The Detection of Glycosphingolipids in Brain Tissue Sections by Imaging Mass Spectrometry Using Gold Nanoparticles
复制标题
DOI：
10.1016/j.jasms.2010.08.002
复制
发表时间：
2010-11-01
影响因子：
3.2
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Goto-Inoue, Naoko;Hayasaka, Takahiro;Setou, Mitsutoshi
鞘糖脂（GSL）是由亲水性碳水化合物链和疏水性神经酰胺部分组成的两亲性分子。它们似乎主要参与生物过程，如细胞增殖，分化和信号传导。为了更详细地研究脑功能的机制，需要一种更高灵敏度的方法来揭示GSL在脑中的分布。在这份报告中，我们描述了一种简单而有效的方法，用于映射的分布和本地化的GSL目前在小鼠脑切片使用纳米粒子辅助激光解吸/电离成像质谱（IMS）。我们已经开发并测试了金纳米颗粒（AuNPs）作为一种新的基质，以最大限度地检测GSL。用烷基胺修饰的AuNPs基质用于检测小鼠脑切片中的各种GSL，例如硫苷脂和神经节苷脂的小分子种类;使用2，5-二羟基苯甲酸（DHB）几乎检测不到这些GSL，2，5-二羟基苯甲酸（DHB）是GSL的常规基质。与DHB基质相比，我们使用AuNP实现了大约20倍更灵敏的GSL检测。我们相信，我们在IMS中使用AuNPs的新方法可以通过次要GSL的分布来分析基本生物学机制和几种疾病。（J Am Soc Mass Spectrom 2010，21，1940-1943）（C）2010年美国质谱学会
Glycosphingolipids (GSLs) are amphiphilic molecules consisting of a hydrophilic carbohydrate chain and a hydrophobic ceramide moiety. They appear to be involved primarily in biological processes such as cell proliferation, differentiation, and signaling. To investigate the mechanism of brain function in more detail, a more highly sensitive method that would reveal the GSL distribution in the brain is required. In this report, we describe a simple and efficient method for mapping the distribution and localization of GSLs present in mouse brain sections using nanoparticle-assisted laser desorption/ionization imaging mass spectrometry (IMS). We have developed and tested gold nanoparticles (AuNPs) as a new matrix to maximize the detection of GSLs. A matrix of AuNPs modified with alkylamine was used to detect various GSLs, such as minor molecular species of sulfatides and gangliosides, in mouse brain sections; these GSLs were hardly detected using 2,5-dihydroxybenzoic acid (DHB), which is the conventional matrix for GSLs. We achieved approximately 20 times more sensitive detection of GSLs using AuNPs compared to a DHB matrix. We believe that our new approach using AuNPs in IMS could lead to a new strategy for analyzing basic biological mechanisms and several diseases through the distribution of minor GSLs. (J Am Soc Mass Spectrom 2010, 21, 1940-1943) (C) 2010 American Society for Mass Spectrometry