Immunoproteomic analysis of the murine antibody response to successful and failed immunization with live anti-Francisella vaccines.
Immunoproteomic analysis of the murine antibody response to successful and failed immunization with live anti-Francisella vaccines.
复制标题
DOI：
10.1016/j.bbrc.2006.06.008
复制
发表时间：
2006-08
影响因子：
3.1
通讯作者：
S. Twine;Mireille D. Petit;Hua Shen;N. Mykytczuk;J. Kelly;J. W. Conlan
中科院分区：
文献类型：
--
作者：
S. Twine;Mireille D. Petit;Hua Shen;N. Mykytczuk;J. Kelly;J. W. Conlan
土拉热弗朗西丝菌土拉热亚种是人类最致命的细菌病原体之一。通过接种活疫苗而不是灭活疫苗，可以在人类和一些但不是全部小鼠品系中诱导针对病原体的保护性免疫。在小鼠中，这种保护主要由CD 4+和CD 8 + T细胞介导。人们认为人类也是如此。然而，成功的疫苗接种可能会产生抗原特异性抗体，这些抗体可以作为保护的相关物。为了验证这一假设，我们检测了成功免疫BALB/c和CH 3/HeN小鼠与不成功免疫C57 BL/6和DBA E2小鼠后诱导的抗体库。tularensis活疫苗株或高度相关亚种F. novicida。结果表明，成功的疫苗接种引发了至少六种蛋白质的抗体，这些蛋白质不能被接种疫苗但未受保护的小鼠的抗血清识别。
Francisella tularensis subspecies tularensis is one of the most virulent of bacterial pathogens for humans. Protective immunity against the pathogen can be induced in humans and some, but not all, mouse strains by vaccination with live, but not killed, vaccines. In mice, this protection is mediated predominantly by CD4+ and CD8+ T cells. This is thought to be the case too for humans. Nevertheless, it is possible that successful vaccination elicits antigen-specific antibodies that can serve as correlates of protection. To test this hypothesis we examined the repertoire of antibodies induced following successful immunization of BALB/c and CH3/HeN mice versus unsuccessful vaccination of C57BL/6 and DBA⧹2 mice with F. tularensis Live Vaccine Strain or following unsuccessful vaccination of BALB/c mice with highly related subspecies, F. novicida. The results showed that successful vaccination elicited antibodies to at least six proteins that were not recognized by antisera from vaccinated but unprotected mice.