Non-invasive identification of proteoglycans and chondrocyte differentiation state by Raman microspectroscopy
Non-invasive identification of proteoglycans and chondrocyte differentiation state by Raman microspectroscopy
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DOI：
10.1002/jbio.201200064
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发表时间：
2013-02-01
影响因子：
2.8
通讯作者：
Schenke-Layland, Katja
中科院分区：
文献类型：
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作者：
Pudlas, Marieke;Brauchle, Eva;Schenke-Layland, Katja
蛋白聚糖（PG）是存在于所有组织和器官中的重要细胞外基质（ECM）组分。这些大分子的病理性重塑可导致严重的疾病，如骨关节炎或类风湿性关节炎。迄今为止，PG相关的ECM改变通常通过侵入性分析方法进行诊断。在这里，我们采用了拉曼显微光谱学，一种基于激光的，无标记的和非破坏性的技术，允许产生的光谱与峰起源于样品内的分子振动，以确定特定的拉曼频带，可以分配给PG在人类和猪软骨样品和软骨细胞。基于非侵入性获得的拉曼光谱，我们进一步揭示，在体外培养的延长导致软骨细胞的表型改变，导致PG合成速率降低和脂质含量的损失。我们的研究结果是第一次证明拉曼显微光谱作为一种分析和潜在的诊断工具，非侵入性的细胞和组织状态监测软骨生物医学研究的适用性。((c)2013 WILEY-VCH Verlag GmbH & Co. KGaA，魏因海姆）
Proteoglycans (PGs) are crucial extracellular matrix (ECM) components that are present in all tissues and organs. Pathological remodeling of these macromolecules can lead to severe diseases such as osteoarthritis or rheumatoid arthritis. To date, PG-associated ECM alterations are routinely diagnosed by invasive analytical methods. Here, we employed Raman microspectroscopy, a laser-based, marker-free and non-destructive technique that allows the generation of spectra with peaks originating from molecular vibrations within a sample, to identify specific Raman bands that can be assigned to PGs within human and porcine cartilage samples and chondrocytes. Based on the non-invasively acquired Raman spectra, we further revealed that a prolonged in vitro culture leads to phenotypic alterations of chondrocytes, resulting in a decreased PG synthesis rate and loss of lipid contents. Our results are the first to demonstrate the applicability of Raman microspectroscopy as an analytical and potential diagnostic tool for non-invasive cell and tissue state monitoring of cartilage in biomedical research. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)