Improvements in skills and knowledge after a comprehensive ELISA teaching course for biotechnology undergraduates
Improvements in skills and knowledge after a comprehensive ELISA teaching course for biotechnology undergraduates
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DOI：
10.1002/bmb.21739
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发表时间：
2023-05
影响因子：
1.4
通讯作者：
Jingmin Yan;Jiangao Chen;X. Mao;Qian Li
中科院分区：
文献类型：
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作者：
Jingmin Yan;Jiangao Chen;X. Mao;Qian Li
酶联免疫吸附试验（ELISA）作为一种普遍和广泛采用的技术，可用于在临床和分析的许多应用中检测和定量小分子。但学生一般都是按照说明书机械地使用商用ELISA试剂盒进行实验，最终制作标准曲线来计算待测样品的浓度，无法理解方法建立的关键因素和过程。本研究系统地介绍了利用病原体特异性抗原，建立一种间接ELISA方法检测类鼻疽伯克霍尔德氏菌的诊断性靶病原体。本课程旨在培养学生的实验技能，提高学生的科学研究知识，充分体现了科学研究与教学的有机结合。学生自主选择感兴趣的诊断抗原靶标，利用基因工程技术获得抗原蛋白，并通过一系列条件优化实验建立了ELISA方法。此外，典型的学生生成的数据，实验方法，并在这项研究中的学生反馈的解释。总体而言，学生能够将抽象知识与实践联合收割机相结合，了解抗原抗体相互作用的原理和应用，从而使他们获得分子生物学技术的实践经验，并学会如何利用这一原理建立检测传染病的ELISA方法。
As a universal and extensively adopted technique, enzyme‐linked immunosorbent assay (ELISA) can be used to detect and quantify small molecules in many applications both clinical and analytical. However, generally, students experiment mechanically using commercial ELISA kits according to the instructions and eventually produce a standard curve to calculate the concentration of the sample to be measured, cannot understand the critical factors and process of method establishment. This study systematically introduced undergraduates to using the pathogen‐specific antigen and establishing an indirect ELISA method to detect the diagnostic target pathogen Burkholderia pseudomallei. This course aimed to develop the experimental skills of the students and improve their scientific research knowledge, which fully embody the organic combination of scientific research and teaching. Students independently selected the diagnostic antigen target of interest, obtained the antigen proteins using genetic engineering techniques, and established an ELISA method through a series of conditional optimization experiments. In addition, typical student‐generated data, experimental methods, and a student feedback interpretation are presented in this study. Overall, the students were able to combine abstract knowledge with practice and understand the principles and applications of antigen–antibody interactions, thus enabling them to gain practical experience in molecular biology techniques, and learn how to use this principle to establish an ELISA method for detecting infectious diseases.