A bone stain for osteoid seams in fresh, unembedded, mineralized bone.
A bone stain for osteoid seams in fresh, unembedded, mineralized bone.
复制标题
用于新鲜、未嵌入、矿化骨中类骨质接缝的骨染色。
作者：
A. Villanueva
将 75-100 μ 厚的新鲜研磨矿化骨切片在 Bone Stain 中染色 90 分钟或 48 小时，Bone Stain 是一种含有固绿 FCF、橙色 G、碱性品红和天蓝色 II 的制剂。然后在流水下研磨去除表面污渍。将切片用 0.1% 氯化泽菲兰（苯扎氯铵）或 0.01% 温和肥皂洗涤，并再次用自来水洗涤，然后用蒸馏水洗涤。接下来，切片在 95% 甲醇中的 0.01% 乙酸中分化，在 95% 乙醇和 100% 乙醇中脱水，在酒精：二甲苯 1:1、1:4、1:9 和 2 种二甲苯中透明，然后永久封固在 Eukitt 封固剂中。骨缝染色为绿色至玉绿色或红色至深红色，不完全矿化的骨红色或橙黄色，以及分界区浅绿色。腔隙、小管、羽状骨、手术伪影和骨膜细胞腔隙低密度版本的壁被染成红色。该方法有助于鉴别诊断人类某些代谢性骨疾病。
Fresh, ground, mineralized bone sections 75-100 μ thick are stained 90 minutes or 48 hours in the Bone Stain, a preparation containing fast green FCF, orange G, basic fuchsin, and azure II. Surface stain is then removed by grinding under running water. Sections are washed in 0.1% zephiran chloride (benzalkonium chloride) or in 0.01% mild soap and again washed in tap water, followed with distilled water. Sections are next differentiated in 0.01% acetic acid in 95% methanol, dehydrated in 95% ethanol and 100% ethanol, cleared in alcohol:xylene 1:1, 1:4, 1:9 and 2 changes of xylol, and then mounted permanently in Eukitt's mounting media.Osteoid seams stain either green to jade green or red to dark red, incompletely mineralized bone red or orange yellow, and the zone of demarcation light green. The walls of lacunae, canaliculae, feathered bone, procedural artifacts and periosteocyte lacunar low-density versions stain red.The method helps in the differential diagnosis of certain metabolic bone diseases in huma...