DELAYED HYPERSENSITIVITY IN VITRO - ITS MEDIATION BY CELL-FREE SUBSTANCES FORMED BY LYMPHOID CELL-ANTIGEN INTERACTION
DELAYED HYPERSENSITIVITY IN VITRO - ITS MEDIATION BY CELL-FREE SUBSTANCES FORMED BY LYMPHOID CELL-ANTIGEN INTERACTION
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DOI：
10.1073/pnas.56.1.72
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发表时间：
1966-01-01
影响因子：
11.1
通讯作者：
DAVID, JR
中科院分区：
文献类型：
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作者：
DAVID, JR
在与特异性抗原孵育期间，敏感的豚鼠淋巴细胞（95%淋巴细胞）在培养基中产生可溶性物质，抑制正常腹膜渗出液细胞的迁移。在与无关抗原孵育或不与抗原孵育的敏感淋巴结细胞的上清液中未发现该物质。当将特异性抗原加入到无抗原孵育24小时的敏感细胞的上清液中时，上清液对正常细胞没有影响。该物质的产生受到嘌呤霉素的抑制。特异性抗原可刺激敏感的淋巴细胞产生可溶性物质，抑制正常腹腔渗出液细胞的体外迁移。抑制物质不可透析，在56 ℃加热30分钟后具有活性。
Sensitive guinea pig lymphoid cells (95% lymphocytes), during incubation with specific antigen, elaborate a soluble substance into the media which inhibits the migration of normal peritoneal exudate cells. The material is not found in supernatants from sensitive lymph node cells incubated with an unrelated antigen, or incubated without antigen. When the specific antigen is added to supernatants from sensitive cells incubated for 24 hr. without antigen, the supernatants have no effect on normal cells. The production of this material is inhibited by puromycin. Specific antigen may stimulate sensitive lymphoid cells to produce soluble materials which inhibit the in vitro migration of normal peritoneal exudate cells. The inhibitory substance is nondialyzable, and active after heating at 56[degree]C for 30 min.