A REVERSIBLE TYPE OF NEURONAL INJURY FOLLOWING ISCHEMIA IN THE GERBIL HIPPOCAMPUS
A REVERSIBLE TYPE OF NEURONAL INJURY FOLLOWING ISCHEMIA IN THE GERBIL HIPPOCAMPUS
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DOI：
10.1161/01.str.17.3.455
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发表时间：
1986-05-01
期刊：
影响因子：
8.3
通讯作者：
SANO, K
中科院分区：
文献类型：
--
作者：
KIRINO, T;TAMURA, A;SANO, K
已知蒙古沙鼠在短暂的前脑缺血后会在海马体中出现迟发性神经元死亡（Brain Res 239：57-69，1982）。研究了戊巴比妥对神经元损伤这一缓慢过程的影响。双侧颈动脉闭塞 5 分钟后，立即注射戊巴比妥（10、20 或 40 mg/kg）。对照动物接受盐水注射。缺血性损伤后 7 天，对动物进行灌注固定，并对海马 CA1 亚区的神经元密度进行计数。当给予戊巴比妥时，CA1区的大多数神经元在缺血性损伤中幸存下来，而对照组的大多数神经元在未经治疗的情况下消失。 20mg/kg组的平均神经元密度为168.2±。 CA1 子场每 1 mm 线性长度为 12.3 (SEM)。 40mg/kg组的密度为181.1±。 14.9。整个对照组的神经元密度为34.3±。 5.1.未手术的正常沙鼠的密度为212.3±。 3.9.这一结果表明“延迟性神经元死亡”的神经元损伤是可逆的。另一方面，当缺血后1小时注射戊巴比妥时，没有显示出效果。 CA1区的细胞变化在初始阶段是可逆的，但似乎很快变得不可逆，而神经元在形态上仍然保持完整。
The Mongolian gerbil is known to develop delayed neuronal death in the hippocampus following brief forebrain ischemia (Brain Res 239: 57-69, 1982). The effect of pentobarbital on this slow process of neuronal damage was examined. Immediately following 5 min of bilateral carotid occlusion, pentobarbital (10, 20, or 40 mg/kg) was injected. The control animals received saline injection. Seven days following ischemic insult, animals were perfusion-fixed and the neuronal density in the hippocampal CA1 subfield was counted. Most of the neurons in the CA1 sector survived ischemic insult when pentobarbital was given, whereas most of control group neurons were lost without the treatment. The average neuronal density of 20 mg/kg group was 168.2 .+-. 12.3 (SEM) per 1 mm linear length of the CA1 subfield. The density in 40 mg/kg group was 181.1 .+-. 14.9. The neuronal density in the whole control group was 34.3 .+-. 5.1. The density of unoperated normal gerbils was 212.3 .+-. 3.9. This result indicates that the neuronal damage of "delayed neuronal death" is reversible. On the other hand, when pentobarbital was injected 1 hr following ischemia, it showed no effect. The cell change in the CA1 sector, reversible at the initial stage, seems to rapidly become irreversible, while neurons still remain intact morphologically.