Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea
Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea
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DOI：
10.1038/ismej.2007.51
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发表时间：
2007-12-01
期刊：
影响因子：
11
通讯作者：
Zhou, Shining
中科院分区：
文献类型：
--
作者：
Lai, Xintian;Cao, Lixiang;Zhou, Shining
为了阐明南海深海沉积物中真菌的多样性，对5个不同沉积物样品的rRNA基因的ITS区进行了扩增和序列分析。共构建了5个ITS文库，随机选取413个克隆，经扩增核糖体DNA限制性酶切分析（ARM-RFLP），将其分为24种酶切图谱。对44个代表性克隆进行ITS序列测定，并与GenBank数据库进行gapped-BLAST比对。系统发育分析表明，该菌株与茎点霉属（Phoma）、洛德酵母属（Lodderomyces）、马拉色菌属（Malassezia）、隐球菌属（Cryptococcus）、绒果霉属（Aciddrocarpon）、大麦属（Hortaea）、毕赤酵母属（Pichia）、曲霉属（Aspergillus）和假丝酵母属（Candida）的ITS序列相似性在71 - 97%之间。其余序列与公共数据库中的任何已知真菌或真菌序列无关。结果表明，甲烷水合物的深海海洋沉积物中含有多种真菌。本文首次报道了南海深海甲烷水合物沉积物中真菌群落的研究。
To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different sediment DNA samples were amplified and phylogenetically analyzed. Total five ITS libraries were constructed and 413 clones selected randomly were grouped into 24 restriction patterns by Amplified Ribosomal DNA Restriction Analysis (ARDRA). ITS sequences of 44 representative clones were determined and compared with the GenBank database using gapped-BLAST. The phylogenetic analysis showed that the ITS sequences (71 - 97% similarity) were similar to those of Phoma, Lodderomyces, Malassezia, Cryptococcus, Cylindrocarpon, Hortaea, Pichia, Aspergillus and Candida. The remaining sequences were not associated to any known fungi or fungal sequences in the public database. The results suggested that methane hydrate-bearing deep-sea marine sediments harbor diverse fungi. This is the first report on fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea.