Effects of quorum sensing molecules of Pseudomonas aeruginosa on organism growth, elastase B production, and primary adhesion to hydrogel contact lenses.
Effects of quorum sensing molecules of Pseudomonas aeruginosa on organism growth, elastase B production, and primary adhesion to hydrogel contact lenses.
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DOI：
10.1097/01.icl.0000146324.28865.e8
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发表时间：
2005-03-01
期刊：
影响因子：
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通讯作者：
Ahearn, Donald
中科院分区：
文献类型：
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作者：
George, Melanie;Pierce, George;Ahearn, Donald
目得：铜绿假单胞菌胞外蛋白酶的产生和生物被膜的结构受到被称为群体感应（QS）因子的酰化高丝氨酸内酯（AHLs）的影响。评价了铜绿假单胞菌的C12-和C4-AHLs和合成呋喃酮信号拮抗剂对铜绿假单胞菌菌株（包括QS突变体）和金黄色葡萄球菌和表皮葡萄球菌的分离株的生长、对水凝胶接触镜的主要粘附和弹性蛋白酶B产生的影响。通过三磷酸腺苷分析检查铜绿假单胞菌对浸泡在AHL和呋喃酮中的晶状体的原发性粘附。弹性蛋白酶B活性的培养物暴露于AHLs和呋喃酮测定由弹性蛋白刚果红assay.Results：酰化高丝氨酸内酯有最大细胞密度或初级粘附没有显着的影响。lasI-rhlI- QS突变体与晶状体的粘附性显著低于其野生型（WT）菌株，无论添加阿勒或呋喃酮。外源AHLs对WT铜绿假单胞菌的弹性蛋白酶产生的影响可以忽略不计，但恢复了QS缺陷突变体的弹性蛋白酶产生。呋喃酮对葡萄球菌具有杀生物作用，但促进了lasI-rhlI- QS突变体的生长。呋喃酮减少WT菌株的弹性蛋白酶生产，但增加QS突变体弹性蛋白酶production.CONCLUSIONS：外源性AHLs和呋喃酮是次要的铜绿假单胞菌的初始粘附到镜头。呋喃酮降低WT铜绿假单胞菌的葡萄球菌生长和弹性蛋白酶产生，但增加QS突变体的生长和弹性蛋白酶产生。数据进一步表明铜绿假单胞菌中的菌株多样性和阿勒系统的复杂性。
PURPOSE: Extracellular proteinase production and biofilm structure of Pseudomonas aeruginosa are affected by acylated homoserine lactones (AHLs) termed quorum sensing (QS) factors. C12- and C4-AHLs of P. aeruginosa and a synthetic furanone signaling antagonist were evaluated for effects on growth, primary adhesion to hydrogel contact lenses, and elastase B production of P. aeruginosa strains, including QS mutants, and isolates of Staphylococcus aureus and Staphylococcus epidermidis.METHODS: Acylated homoserine lactones and furanone were added to cultures of bacteria at various growth stages. Primary adhesion of P. aeruginosa to lenses soaked in AHLs and furanone was examined by adenosine triphosphate analysis. Elastase B activity of cultures exposed to AHLs and furanone was determined by an elastin congo red assay.RESULTS: Acylated homoserine lactones had no significant effects on maximal cell densities or primary adhesion. Adhesion of a lasI-rhlI- QS mutant to lenses was significantly lower than its wild-type (WT) strain, regardless of added AHL or furanone. Exogenous AHLs had negligible effects on elastase production of WT P. aeruginosa but restored elastase production in QS-deficient mutants. The furanone was biocidal for staphylococci but enhanced growth of the lasI-rhlI- QS mutant. The furanone decreased elastase production of WT strains but increased QS mutant elastase production.CONCLUSIONS: Exogenous AHLs and the furanone were of minor importance in initial adhesion of P. aeruginosa to lenses. The furanone decreased staphylococci growth and elastase production of WT P. aeruginosa but increased growth and elastase production of QS mutants. The data further indicate strain diversity in P. aeruginosa and complexity of AHL systems.