Current Status and Strategy of microRNA Research for Cartilage Development and Osteoarthritis Pathogenesis.
Current Status and Strategy of microRNA Research for Cartilage Development and Osteoarthritis Pathogenesis.
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DOI：
10.11005/jbm.2016.23.3.121
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发表时间：
2016-08
影响因子：
--
通讯作者：
Asahara H
中科院分区：
文献类型：
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作者：
Asahara H
MicroRNA (miRNA) 是一种小型（~21 个核苷酸）非编码 RNA，在软骨内骨化、关节软骨稳态和关节炎发病机制中发挥着重要作用。对软骨特异性或软骨相关 miRNA 的全面遗传分析提供了有关软骨发育、体内平衡和相关疾病的新信息。最先进的组合方法，包括用于靶向 miRNA 的转录激活因子样效应核酸酶 (TALEN)/成簇规则间隔短回文重复序列 (CRISPR) 技术，以及通过交联免疫沉淀分离的 RNA 高通量测序以识别目标信使 RNA，应用于确定复杂的 miRNA 网络和 miRNA 依赖性软骨调节。使用涉及软骨特异性 miRNA 的先进药物输送系统将加速这些新发现在关节炎治疗中的应用。
MicroRNAs (miRNAs), which are small (~21 nucleotides) non-coding RNAs, are important players in endochondral ossification, articular cartilage homeostasis, and arthritis pathogenesis. Comprehensive and genetic analyses of cartilage-specific or cartilage-related miRNAs have provided new information on cartilage development, homeostasis, and related diseases. State-of-the-art combinatorial approaches, including transcription-activator like effector nuclease (TALEN)/clustered regularly interspaced short palindromic repeats (CRISPR) technique for targeting miRNAs and high-throughput sequencing of RNA isolated by cross-linking immunoprecipitation for identifying target messenger RNAs, should be used to determine complex miRNA networks and miRNA-dependent cartilage regulation. Use of advanced drug delivery systems involving cartilage-specific miRNAs will accelerate the application of these new findings in arthritis therapy.