Digital dermatitis treponemes associated with a severe foot disease in dairy goats
Digital dermatitis treponemes associated with a severe foot disease in dairy goats
复制标题
与奶山羊严重足部疾病相关的指趾皮炎密螺旋体
DOI：
--
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
J. Duncan
中科院分区：
文献类型：
--
作者：
L. E. Sullivan;N. Evans;S. Clegg;Stuart D. Carter;J. E. Horsfield;D. Grove;J. Duncan
英国奶山羊群进行了评估后，一个严重的跛行问题的病因不明的报告。对15只随机选择的跛足山羊进行了跛足患病率估计和个体临床检查。15只动物的足部病变与绵羊的传染性绵羊指皮炎（CODD）非常相似。8只山羊检查了典型的CODD病变，7只山羊表现出更严重的CODD，鞋底欠跑。从足部病变处获得10个活检样本，并通过PCR检测先前分离的三个指皮炎（DD）密螺旋体群，并尝试培养密螺旋体。90%的活检样本对中等密螺旋体/类文森特密螺旋体和类噬菌密螺旋体DD螺旋体呈阳性，80%对足密螺旋体呈阳性。从50%的病变样本中成功分离出螺旋体。经鉴定，有3个菌株属于T.噬菌体样螺旋体，2株鉴定为T.脚与从牛指皮炎和CODD病变中分离出的密螺旋体相似的密螺旋体的频繁分离以及在绝大多数病变中鉴定出这些DD相关的密螺旋体类型支持以下假设：这种新的足部疾病与DD密螺旋体感染相关，并且鉴于与CODD的相似性，它表明了因果关系。
A UK dairy goat herd was assessed after reports of a severe lameness problem of unknown aetiology. A lameness prevalence estimate was produced and individual clinical examination of 15 randomly selected lame goats was performed. Fifteen animals had foot lesions closely resembling contagious ovine digital dermatitis (CODD) in sheep. Eight of the goats examined presented with typical CODD lesions and seven showed what appeared to be a more severe CODD with under-running of the sole. Ten biopsy samples were obtained from the foot lesions and tested by PCR for the three previously isolated digital dermatitis (DD) Treponema phylogroups and culture of treponemes was attempted. Ninety per cent of the biopsy samples were positive for Treponema medium/Treponema vincentii-like spirochaetes and Treponema phagedenis-like DD spirochaetes and 80per cent were positive for Treponema pedis. Spirochaetes were successfully isolated from 50 per cent of lesion samples. Three isolates were identified as belonging to the T. phagedenis-like spirochaetes and two were identified as T. pedis. The frequent isolation of similar treponemes to those isolated from bovine digital dermatitis and CODD lesions and the identification of these DD-associated phylotypes in the vast majority of lesions support the hypothesis that this novel foot condition is associated with infection by DD treponemes, and given the similarities to CODD, it suggests a causal role.
DNA 序列分析。
影响因子：
2.3
作者：
Weir,BS
通讯作者：
Weir,BS