An analysis of mobile genetic elements in three Plasmodium species and their potential impact on the nucleotide composition of the P. falciparum genome.
An analysis of mobile genetic elements in three Plasmodium species and their potential impact on the nucleotide composition of the P. falciparum genome.
复制标题
DOI：
10.1186/1471-2164-7-282
复制
发表时间：
2006-11-04
期刊：
影响因子：
4.4
通讯作者：
Coetzer TL
中科院分区：
文献类型：
--
作者：
Durand PM;Oelofse AJ;Coetzer TL
恶性疟原虫、约氏疟原虫和间日疟原虫的完整基因组序列揭示了一些不同寻常的特征。恶性疟原虫是迄今为止测得的AT含量最高的，在某些地区超过90%。相比之下，约氏疟原虫为~77%，间日疟原虫为~55%。这些发现的进化原因尚不清楚。可移动的遗传元件对基因组进化有相当大的影响，但对疟原虫中的这些元件还没有进行彻底的调查。因此，我们对这三种疟原虫的这些成分及其衍生物进行了全面的基因组分析。利用生物信息学方法进行全基因组分析。在间日疟原虫、约氏疟原虫和恶性疟原虫中分别鉴定出40个具有转座元件特征的潜在蛋白质编码序列、8个编码序列和6个编码序列。对恶性疟原虫六个开放阅读框的进一步研究表明，只有一个可能是一个活跃的可移动遗传元件。在这三个物种中发现的大多数开放阅读框都是假设的蛋白质。有些代表带有注释的宿主蛋白，例如约氏疟原虫和恶性疟原虫中可能的端粒酶逆转录酶基因。本研究中发现的间日疟原虫开放阅读框之一与端粒酶逆转录酶相似，我们认为它是该基因的同源基因。在所研究的三种疟原虫中，可移动遗传元件的频率存在差异。尽管全基因组分析方法存在局限性，但人们很容易推测，可移动的遗传元件可能是恶性疟原虫基因组组成偏差背后的驱动力。
The completed genome sequences of the malaria parasites P. falciparum, P. y. yoelii and P. vivax have revealed some unusual features. P. falciparum contains the most AT rich genome sequenced so far – over 90% in some regions. In comparison, P. y. yoelii is ~77% and P. vivax is ~55% AT rich. The evolutionary reasons for these findings are unknown. Mobile genetic elements have a considerable impact on genome evolution but a thorough investigation of these elements in Plasmodium has not been undertaken. We therefore performed a comprehensive genome analysis of these elements and their derivatives in the three Plasmodium species. Whole genome analysis was performed using bioinformatic methods. Forty potential protein encoding sequences with features of transposable elements were identified in P. vivax, eight in P. y. yoelii and only six in P. falciparum. Further investigation of the six open reading frames in P. falciparum revealed that only one is potentially an active mobile genetic element. Most of the open reading frames identified in all three species are hypothetical proteins. Some represent annotated host proteins such as the putative telomerase reverse transcriptase genes in P. y. yoelii and P. falciparum. One of the P. vivax open reading frames identified in this study demonstrates similarity to telomerase reverse transcriptase and we conclude it to be the orthologue of this gene. There is a divergence in the frequencies of mobile genetic elements in the three Plasmodium species investigated. Despite the limitations of whole genome analytical methods, it is tempting to speculate that mobile genetic elements might have been a driving force behind the compositional bias of the P. falciparum genome.
登录
查看更多内容
影响因子：
14.9
作者：
Kuhlmann M;Borisova BE;Kaller M;Larsson P;Stach D;Na J;Eichinger L;Lyko F;Ambros V;Söderbom F;Hammann C;Nellen W
通讯作者：
Nellen W
影响因子：
14.9
作者：
Figueiredo LM;Rocha EP;Mancio-Silva L;Prevost C;Hernandez-Verdun D;Scherf A
通讯作者：
Scherf A
影响因子：
5.6
作者：
KROGH, A;BROWN, M;HAUSSLER, D
通讯作者：
HAUSSLER, D
影响因子：
11.4
作者：
XIONG, Y;EICKBUSH, TH
通讯作者：
EICKBUSH, TH
影响因子：
56.9
作者：
Lingner, J;Hughes, TR;Cech, TR
通讯作者：
Cech, TR