Psoriatic scales:: a promising source for the isolation of human skin-derived antimicrobial proteins
Psoriatic scales:: a promising source for the isolation of human skin-derived antimicrobial proteins
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DOI：
10.1189/jlb.0704409
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发表时间：
2005-04-01
影响因子：
5.5
通讯作者：
Schröder, JM
中科院分区：
文献类型：
--
作者：
Harder, J;Schröder, JM
银屑病是一种慢性、过度增殖性和非感染性皮肤病，其患者的皮肤感染比预期的要少得多。这一观察结果使我们提出了一个假设，即抗菌蛋白形式的局部“化学屏障”为银屑病皮肤提供了抵抗感染的能力。随后，我们开始系统分析银屑病规模提取物中的体外抗菌活性蛋白。一种生物化学方法，结合严格的纯化和表征以及抗微生物测试，鉴定了许多主要是新的人类抗生素肽和蛋白质。在这篇综述中，我们将重点放在最突出的抗菌蛋白质在银屑病规模提取物，我们确定为S100蛋白银屑病，人类β-防御素2（hBD-2），核糖核酸酶7，溶菌酶，和人类中性粒细胞防御素1-3。除了这些皮肤，抗菌蛋白，只有少数人，包括hBD-3，已被确定。大量的次要抗微生物蛋白质等待进一步的结构表征。
Patients with psoriasis, a chronic, hyperproliferative and noninfectious skin disease, suffer surprisingly fewer cutaneous infections than would be expected. This observation led us to the hypothesis that a local "chemical shield" in the form of antimicrobial proteins provides psoriatic skin with resistance against infection. We subsequently began a systematic analysis of in vitro antimicrobially active proteins in psoriatic-scale extracts. A biochemical approach with rigorous purification and characterization combined with antimicrobial testing identified a number of mostly new human antibiotic peptides and proteins. In this review, we will focus on the most prominent antimicrobial proteins in psoriaticscale extracts, which we identified as the S100-protein psoriasin, human beta-defensin 2 (hBD-2), RNase 7, lysozyme, and human neutrophil defensin 1-3. Apart from these cutaneous, antimicrobial proteins, only a few others, including hBD-3, have been characterized. A great number of minor antimicrobial proteins await further structural characterization.