Physical-chemical stability and in vitro digestibility of hybrid nanoparticles based on the layer-by-layer assembly of lactoferrin and BSA on liposomes
Physical-chemical stability and in vitro digestibility of hybrid nanoparticles based on the layer-by-layer assembly of lactoferrin and BSA on liposomes
复制标题
基于乳铁蛋白和BSA在脂质体上层层组装的杂化纳米粒子的物理化学稳定性和体外消化率
DOI：
10.1039/c7fo00308k
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发表时间：
2017-04-01
期刊：
影响因子：
6.1
通讯作者：
Liu, Jianhua
中科院分区：
文献类型：
--
作者：
Liu, Weilin;Kong, Youyu;Liu, Jianhua
通过静电沉积法将正性牛血清白蛋白（BSA）和负性乳铁蛋白（LF）复合到阴离子纳米脂质体（NLs）表面，制备了杂化纳米粒子。所得颗粒的累积尺寸为156.27 +/-11.0 nm，并且负电荷减少。透射电子显微镜（TEM）显示，混合粒子形成一个光滑的球形复合物后，球状蛋白沉积。通过热处理、pH值改变和长期贮存后的粒径分布和表面电荷的观察发现，包被有聚电解质BSA和LF的脂质体的稳定性明显优于裸脂质体。在体外胃肠道消化试验中，单层包衣和双层包衣的纳米粒在微观结构（TEM）和模型药物（钙黄绿素）释放速率方面的变化相似，均上级未包衣的纳米粒。这些结果表明，通过静电相互作用将BSA和LF聚电解质包覆在脂质体表面的杂化纳米粒可以提高脂质体的稳定性，并为食品和营养领域控制理化和消化稳定性的功能性分子递送系统的制备提供了一定的启示。
Hybrid nanoparticles were fabricated by the electrostatic deposition of positive bovine serum albumin (BSA) and negative lactoferrin (LF) onto the surface of anionic nanoliposomes (NLs). The resulting particles had a cumulative size of 156.27 +/- 11.0 nm and decreased in negative charge. Transmission electron microscopy (TEM) revealed that the hybrid particles formed a smooth and spherical polyelectrolyte complex after globular protein deposition. Observations in size distribution and surface charge after heat treatment, pH alteration and long-term storage found that the particles coated with layers of polyelectrolytes, BSA and LF, had obviously better stability than the bare liposomes. In an in vitro gastrointestinal digestion study, monolayer coated NLs (LF-NLs) and double-layer coated NLs (BSA-LF-NLs) had similar changes in microstructure (TEM) and the release rate of model cargos (calcein), which were superior to the uncoated NLs. These results indicated that hybrid nanoparticles coated with the polyelectrolytes of BSA and LF on the surface of liposomes by electrostatic interaction may improve liposomal stability, and showed some implications for the fabrication of functional molecular delivery systems to control physical-chemical and digestion stability in food and nutrition areas.