Biological glucose metabolism regulated peptide self-assembly as a simple visual biosensor for glucose detection.
Biological glucose metabolism regulated peptide self-assembly as a simple visual biosensor for glucose detection.
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DOI：
10.1002/marc.201100689
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发表时间：
2012-03
影响因子：
4.6
通讯作者：
Xiaoding Xu;Bing-Bing Lin-Bing;Jun Feng;Ya Wang;Si-Xue Cheng;Xianzheng Zhang;R. Zhuo
中科院分区：
文献类型：
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作者：
Xiaoding Xu;Bing-Bing Lin-Bing;Jun Feng;Ya Wang;Si-Xue Cheng;Xianzheng Zhang;R. Zhuo
在体外模拟葡萄糖氧化酶（GOx）介导的葡萄糖代谢，并用于调节基于肽的构建块的自组装。在这个新的刺激响应自组装系统中，两个基于肽的构建块，分别具有天冬氨酸（胶凝因子1）和赖氨酸（胶凝因子2）残基的设计和制备。当将葡萄糖和GOx加入到凝胶因子1的水溶液或凝胶因子2的自组装纤维状水凝胶中构建葡萄糖代谢体系时，代谢产物（葡萄糖酸）可以触发肽分子的质子化，并诱导凝胶因子1（溶胶-凝胶）和凝胶因子2（凝胶-溶胶）的相变。由于这种葡萄糖代谢调节肽的自组装是建立在葡萄糖的氧化上的，因此它可以作为一种简单的视觉传感器用于葡萄糖的检测。
A glucose oxidase (GOx)-mediated glucose metabolism was in vitro mimicked and employed to regulate the self-assembly of peptide-based building blocks. In this new stimuli-responsive self-assembly system, two peptide-based building blocks, respectively, having aspartic acid (gelator 1) and lysine (gelator 2) residues were designed and prepared. When adding glucose and GOx to the aqueous solution of gelator 1 or the self-assembled fibrillar hydrogel of gelator 2 to construct glucose metabolism system, the metabolic product (gluconic acid) can trigger the protonation of the peptide molecules and induce the phase transitions of gelators 1 (sol-gel) and 2 (gel-sol). Because this glucose metabolism regulated peptide self-assembly is built on the oxidation of glucose, it can be used as a simple visual biosensor for glucose detection.