In vitro susceptibility of invasive isolates of Candida spp. to anidulafungin, caspofungin, and micafungin:: Six years of global surveillance
In vitro susceptibility of invasive isolates of Candida spp. to anidulafungin, caspofungin, and micafungin:: Six years of global surveillance
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DOI：
10.1128/jcm.01901-07
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发表时间：
2008-01-01
影响因子：
9.4
通讯作者：
Diekema, D. J.
中科院分区：
文献类型：
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作者：
Pfaller, M. A.;Boyken, L.;Diekema, D. J.
棘白菌素正越来越多地用作侵袭性念珠菌病的治疗。前瞻性哨兵监测侵入性念珠菌属分离株中棘白菌素体外耐药性的出现。我们测定了阿尼芬净、卡泊芬净和米卡芬净对5,346株侵袭性（血流或无菌部位）念珠菌分离株的体外活性。从2001年1月1日至2006年12月31日，从全球90多个医疗中心收集。我们根据CLSI M27-A2方法进行了药敏试验，并使用RPMI 1640肉汤、24小时孵育和显著抑制终点测定MIC。在5，346株侵袭性念珠菌分离株中，种属分布为54%的C。白色念珠菌14%。近平滑型C.光叶藓C. glabrata，12%; tropicalis，3% C. krusei，1% C.季也蒙念珠菌属（guilliermondii）和2%其它念珠菌属（Candida spp.）总体而言，所有三种棘白菌素对念珠菌都非常有效：阿尼芬净（MIC 50，0.06 μ g/ml; MIC 90，2 μ g/ml）、卡泊芬净（MIC 50，0.03 μ g/ml; MIC 90，0.25 μ g/ml）、米卡芬净（MIC 50，0.015 μ g/ml; MIC 90，2 μ g/ml）。99%以上的菌株被抑制
The echinocandins are being used increasingly as therapy for invasive candidiasis. Prospective sentinel surveillance for the emergence of in vitro resistance to the echinocandins among invasive Candida sp. isolates is indicated. We determined the in vitro activities of anidulafungin, caspofungin, and micafungin against 5,346 invasive (bloodstream or sterile-site) isolates of Candida spp. collected from over 90 medical centers worldwide from I January 2001 to 31 December 2006. We performed susceptibility testing according to the CLSI M27-A2 method and used RPMI 1640 broth, 24-h incubation, and a prominent inhibition endpoint for determination of the MICs. Of 5,346 invasive Candida sp. isolates, species distribution was 54% C. albicans, 14% C. parapsilosis, 14% C. glabrata, 12% C. tropicalis, 3% C. krusei, 1% C. guilliermondii, and 2% other Candida spp. Overall, all three echinocandins were very active against Candida: anidulafungin (MIC50, 0.06 mu g/ml; MIC90, 2 mu g/ml), caspofungin (MIC50, 0.03 mu g/ml; MIC90, 0.25 mu g/ml), micafungin (MIC50, 0.015 mu g/ml; MIC90, 2 mu g/ml). More than 99% of isolates were inhibited by