Diurnal rhythm of growth hormone-independent binding protein for insulin-like growth factors in human plasma.
Diurnal rhythm of growth hormone-independent binding protein for insulin-like growth factors in human plasma.
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人血浆中胰岛素样生长因子的生长激素独立结合蛋白的昼夜节律。
DOI：
--
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发表时间：
1987
影响因子：
5.8
通讯作者：
C. Cowell
中科院分区：
文献类型：
--
作者：
R. Baxter;C. Cowell
羊水中主要的胰岛素样生长因子（IGF）结合蛋白（BP-28）产生抗体，用RIA法测定血浆中交叉反应蛋白的水平。高效渗透色谱法测定血浆BP-28的表观免疫反应性为35000 mol wt。对15名生长激素分泌活性范围较宽的儿童每小时取样12或24小时，血浆BP-28水平显示出明显的昼夜周期，在2400至600小时之间上升10至20倍，达到50-500微克/升的峰值，然后在1200小时降至基础水平（0-40微克/升）。同一受试者的血浆GH水平每隔20分钟测量一次。在高、正常、矮或GH缺乏儿童中，峰值或基础BP-28水平与实足年龄、骨龄、夜间平均或峰值GH分泌、相对身高或相对生长速度均无显著相关性。用RIA测定53,000 mol wt gh依赖性igf结合蛋白（BP-53）的血浆蛋白没有每日变化。通过用IGF示踪剂孵育血浆和用抗BP-28抗血清沉淀BP-28-IGF复合物来测定血浆中BP-28的IGF结合活性，结果与早晨BP-28免疫反应性升高密切相关。免疫可沉淀的BP-28结合IGF- i和IGF- ii示踪剂，明显倾向于IGF- i，未标记的IGF- i比IGF- ii更有效地取代任何一种示踪剂IGF。我们得出结论，儿童血浆BP-28水平具有明显的昼夜节律，与生长激素分泌活性无关。
An antibody raised against the major insulin-like growth factor (IGF)-binding protein in amniotic fluid (BP-28) was used to measure the levels of a cross-reacting protein in human plasma by RIA. Plasma BP-28 immunoreactivity had an apparent mol wt of 35,000 by high performance permeation chromatography. Sampled hourly for 12- or 24-h periods in 15 children with a wide range of GH secretory activity, plasma BP-28 levels showed a marked diurnal cycle, rising 10- to 20-fold between 2400 and 0600 h to a peak of 50-500 micrograms/L, then falling to basal levels (0-40 micrograms/L) by 1200 h. Plasma GH levels were measured at 20-min intervals in the same subjects. Neither peak nor basal BP-28 levels were significantly associated with chronological age, bone age, mean or peak nocturnal GH secretion, relative height, or relative growth velocity in tall, normal, short, or GH-deficient children. Plasma proteins measured in a RIA for 53,000 mol wt GH-dependent IGF-binding protein (BP-53) did not vary diurnally. The IGF-binding activity of plasma BP-28, measured by incubating plasma with IGF tracer and precipitating the BP-28-IGF complex with anti-BP-28 antiserum, closely paralleled the morning rise in BP-28 immunoreactivity. Immunoprecipitable BP-28 bound both IGF-I and IGF-II tracers, with a clear preference for IGF-I, and unlabeled IGF-I was more effective than IGF-II in displacing either tracer IGF. We conclude that plasma BP-28 levels in children have a marked diurnal rhythm which is unrelated to GH secretory activity.
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DOI：
10.1210/jcem-53-1-100
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DOI：
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DOI：
--
发表时间：
1986
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