Establishment of an in vitro culture system for chicken preblastodermal cells
Establishment of an in vitro culture system for chicken preblastodermal cells
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DOI：
10.1002/mrd.20441
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发表时间：
2006-04-01
影响因子：
2.5
通讯作者：
Han, JY
中科院分区：
文献类型：
--
作者：
Park, HJ;Park, TS;Han, JY
为了开发鸡多能细胞的替代来源，我们研究了（1）未分化的前胚盘细胞是否可以在体外传代培养一段时间，以及（2）传代培养如何影响前胚盘细胞的生理特性。前胚盘细胞的平均数量在V期胚胎中为2，397个，在VII期胚胎中为36，345个; X期胚胎平均有53，857个胚盘细胞。细胞平均直径显著减小（70.63-18.83 μ m; P
To develop an alternative source for chicken pluripotent cells, we examined (1) whether undifferentiated preblastodermal cells could be subcultured in vitro for an extended period and (2) how subculturing affected the physiological properties of preblastodermal cells. The average number of preblastodermal cells was 2,397 in stage V embryos and 36,345 in stage VII embryos; stage X embryos had an average of 53,857 blastodermal cells. The average cell size decreased significantly (70.63-18.83 mu m in diameter; P