Wireless capsule for autofluorescence detection in biological systems
Wireless capsule for autofluorescence detection in biological systems
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DOI：
10.1016/j.snb.2013.03.037
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发表时间：
2013-12-01
影响因子：
8.4
通讯作者：
Cumming, David R. S.
中科院分区：
文献类型：
--
作者：
Al-Rawhani, Mohammed A.;Beeley, James;Cumming, David R. S.
组织自体荧光（AF）的变化可以用来检测肠癌的早期症状，然而目前的内窥镜AF系统只能检查食管和大肠。我们提出了一种能够从哺乳动物肠道组织中诱导和检测AF的药丸的设计、制造和测试。该原型包括一个专用集成电路（ASIC），照明LED，光学滤波器，以尽量减少传感器响应串扰的照明波长，脉冲计数器/控制单元和无线电发射器。该ASIC实现了单光子雪崩二极管探测器（SPAD）和集成高压（高达37.9 V）电荷泵电源。SPAD被偏置到高于其击穿电压以在盖革模式下操作，并且在465 nm处呈现检测效率峰值，其充分接近于520 +/-10 nm的人体组织自体荧光的峰值。ASIC采用商用高压CMOS工艺制造。整个器件的平均功耗仅为21.4 mW。已实施的系统的特征在于对控制的荧光团的解决方案，并在体外与生物样品进行测试。它已被证明是能够诱导和检测荧光的荧光团溶液浓度低至1 nM，并与哺乳动物的肠道组织。(C)2013爱思唯尔有限公司版权所有。
Variations in tissue autofluorescence (AF) can be exploited to detect early signs of intestinal cancer, however current endoscopic AF systems are only able to inspect the oesophegus and large intestine. We present the design, fabrication and testing of a pill capable of inducing and detecting AF from mammalian intestinal tissue. The prototype comprises an application specific integrated circuit (ASIC), illumination LED, optical filters to minimise sensor response to crosstalk from the illumination wavelength, a pulse counter/control unit and a radio transmitter. The ASIC implements a single photon avalanche diode detector (SPAD), and integrated high voltage (up to 37.9 V) charge pump power supply. The SPAD is biased above its breakdown voltage to operate in Geiger mode, and exhibits a detection efficiency peak at 465 nm, sufficiently close to human tissue autofluorescence's peak of 520 +/- 10 nm. The ASIC was fabricated using a commercial high-voltage CMOS process. The complete device uses an average power of only 21.4 mW. The implemented system has been characterised against controlled solutions of fluorophores and tested in vitro with biological samples. It has been proven to be capable of inducing and detecting fluorescence in fluorophore solution concentration as low as 1 nM, and with mammalian intestinal tissue. (C) 2013 Elsevier B.V. All rights reserved.