The In Vivo Genotoxic Effects of Carvacrol and Thymol in Rat Bone Marrow Cells
The In Vivo Genotoxic Effects of Carvacrol and Thymol in Rat Bone Marrow Cells
复制标题
DOI：
10.1002/tox.20380
复制
发表时间：
2008-12-01
影响因子：
4.5
通讯作者：
Rencuzogullari, Eyyup
中科院分区：
文献类型：
--
作者：
Azirak, Sebile;Rencuzogullari, Eyyup
本研究旨在探讨香芹酚和百里酚对大鼠骨髓细胞的体内遗传毒性作用。在本研究中，香芹酚（10、30、50和70 mg/kg b.w.）和百里酚（40、60、80和100 mg/kg b.w.）在所有给药期（6、12和24 h），与腹膜内给药大鼠的骨髓细胞中的对照组相比，显著诱导了结构和总染色体异常（CA）。在最高浓度下，香芹酚和百里酚均显示出与阳性对照尿烷相似的对结构CA和总CA百分比的诱导作用，但香芹酚处理6 h（70 mg/kg b.w.和100 mg/kg b.w.，分别）。此外，与对照相比，香芹酚在所有浓度下均诱导CA数值，在两个最高浓度（50和70 mg/kg b.w.）与溶剂对照相比。麝香草酚还诱导了CA的数值，尤其是在最高浓度（100 mg/kg b.w.）在所有治疗阶段。结果表明，香芹酚和麝香草酚对结构、数量和总CA的诱导均存在剂量依赖性效应。香芹酚和麝香草酚在所有浓度和处理时间与对照相比，有丝分裂指数（MI）降低。香芹酚在70 mg/kg b.w.时显示出与EC相似的降低MI的效果。持续6 h，30和50 mg/kg b.w. 12小时和所有浓度下24小时处理期。在60、80和100 mg/kg b.w.剂量下，百里酚也显示出与氨基甲酸乙酯（氨基甲酸乙酯，EC）相似的降低MI的作用。6小时和所有浓度下24小时处理期。供试品以剂量依赖性方式降低MI。(C)2008 Wiley Periodicals，Inc.环境毒理学23：728-735，2008年。
The aim of this study was to investigate the in vivo genotoxic effects of carvacrol and thymol in bone marrow cells of rats. In the present study, both carvacrol (10, 30, 50, and 70 mg/kg b.w.) and thymol (40, 60, 80, and 100 mg/kg b.w.) significantly induced the structural and total chromosome abnormalities (CA) for all treatment periods (6, 12, and 24 h) when compared with control in bone marrow cells of rats intraperitonally administered. Both carvacrol and thymol showed similar effects with the positive control urethane on induction of the percentage of structural and total CA at the highest concentrations except the effects of carvacrol for 6 h treatment (70 mg/kg b.w. and 100 mg/kg b.w., respectively). In addition, carvacrol induced the numerical CA at all concentrations when compared to control and at two highest concentrations (50 and 70 mg/kg b.w.) when compared to solvent control. Thymol also induced the numerical CA especially at the highest concentration (100 mg/kg b.w.) for all treatment periods. It was shown that there was a dose-dependent effect on induction of structural, numerical and total CA for both carvacrol and thymol. Carvacrol and thymol decreased the mitotic index (MI) in all the concentrations and treatment times when compared with control. Carvacrol showed the similar effects with EC on decreasing the MI at 70 mg/kg b.w. for 6 h, at 30 and 50 mg/kg b.w. for 12 h and at all concentrations for 24 h treatment periods. Thymol also showed a similar effect with urethane (ethyl carbamate, EC) on decreasing the MI at 60, 80, and 100 mg/kg b.w. for 6 h and at all concentrations for 24 h treatment periods. Test substances decreased the MI in a dose-dependent manner. (C) 2008 Wiley Periodicals, Inc. Environ Toxicol 23: 728-735, 2008.