Genetic networks responsive to sodium butyrate in colonic epithelial cells
Genetic networks responsive to sodium butyrate in colonic epithelial cells
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DOI：
10.1016/j.febslet.2006.04.048
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发表时间：
2006-05-29
期刊：
影响因子：
3.5
通讯作者：
Kondo, Takashi
中科院分区：
文献类型：
--
作者：
Tabuchi, Yoshiaki;Takasaki, Ichiro;Kondo, Takashi
我们进行了微阵列和计算基因网络分析，以确定详细的机制，丁酸钠（SB）诱导细胞生长停滞和分化的小鼠结肠上皮MCE 301细胞。在用2mM SB处理的细胞中鉴定了2604个差异表达的探针组，并将其分为四组。其中，逐渐增加组和逐渐显著减少组分别包含细胞发育和细胞周期的遗传网络或脂肪酸生物合成和嘧啶代谢的典型途径。本研究结果为了解SB在结肠上皮细胞中作用的详细分子机制提供了基础。(c)2006年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells treated with 2 mM SB and were classified into four groups. Of these, the gradually increased group and the gradually and remarkably decreased group contained the genetic networks for cellular development and cell cycles or canonical pathways for fatty acid biosynthesis and pyrimidine metabolism, respectively. The present results provide a basis for understanding the detailed molecular mechanisms of action of SB in colonic epithelial cells. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.