Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: Roles of TRAF3 and TRAF5.
Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: Roles of TRAF3 and TRAF5.
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DOI：
10.1016/j.bbrc.2015.10.010
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发表时间：
2015-11
影响因子：
3.1
通讯作者：
Yuki Chiba;T. Matsumiya;T. Satoh;Ryo Hayakari;Ken Furudate;F. Xing;H. Yoshida;K. Tanji;H. Mizukami;T. Imaizumi;E. Ito
中科院分区：
文献类型：
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作者：
Yuki Chiba;T. Matsumiya;T. Satoh;Ryo Hayakari;Ken Furudate;F. Xing;H. Yoshida;K. Tanji;H. Mizukami;T. Imaizumi;E. Ito
在病毒感染时，细胞质病毒传感器视黄酸诱导基因-I（RIG-I）识别病毒RNA以激活抗病毒信号传导以诱导I型干扰素（IFN）。RIG-I样受体（RLR）以组织特异性方式激活抗病毒信号传导。呼吸系统中抗病毒信号传导的分子机制仍不清楚。我们研究了下呼吸道（LRT）中的抗病毒信号，这是许多有害病毒感染的部位。LRT的上皮细胞可大致分为两类：支气管上皮细胞（BEC）和肺泡上皮细胞（AEC）。这两种细胞类型表现出不同的表型;因此，我们假设这些细胞可能在抗病毒先天免疫中发挥不同的作用。我们发现BECs比AECs具有更高的抗病毒活性。TNF受体相关因子3（TRAF 3）已被证明是RLR信号传导中的关键分子。检查了TRAF 3和TRAF 5在LRT中的表达水平，它们具有几乎相同的保守结构域。我们发现，支气管中TRAF 3和TRAF 5的表达水平最高。这些发现表明支气管在LRT抗病毒先天免疫中的重要性，并表明TRAF 3和TRAF 5可能有助于RLR信号传导。
Upon viral infection, the cytoplasmic viral sensor retinoic acid-inducible gene-I (RIG-I) recognizes viral RNA to activate antiviral signaling to induce type I interferon (IFN). RIG-I-like receptors (RLRs) activate antiviral signaling in a tissue-specific manner. The molecular mechanism underlying antiviral signaling in the respiratory system remains unclear. We studied antiviral signaling in the lower respiratory tract (LRT), which is the site of many harmful viral infections. Epithelial cells of the LRT can be roughly divided into two groups: bronchial epithelial cells (BECs) and pulmonary alveolar epithelial cells (AECs). These two cell types exhibit different phenotypes; therefore, we hypothesized that these cells may play different roles in antiviral innate immunity. We found that BECs exhibited higher antiviral activity than AECs. TNF receptor-associated factor 3 (TRAF3) has been shown to be a crucial molecule in RLR signaling. The expression levels of TRAF3 and TRAF5, which have conserved domains that are nearly identical, in the LRT were examined. We found that the bronchus exhibited the highest expression levels of TRAF3 and TRAF5 in the LRT. These findings suggest the importance of the bronchus in antiviral innate immunity in the LRT and indicate that TRAF3 and TRAF5 may contribute to RLR signaling.