Taking tissue-engineering principles into theater: augmentation of impacted allograft with human bone marrow stromal cells.
Taking tissue-engineering principles into theater: augmentation of impacted allograft with human bone marrow stromal cells.
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将组织工程原理带入临床：用人类骨髓基质细胞增强受影响的同种异体移植物。
DOI：
10.2217/17460751.1.5.685
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发表时间：
2006
影响因子：
2.7
通讯作者：
R. Oreffo
中科院分区：
文献类型：
--
作者：
S. Tilley;B. Bolland;K. Partridge;A. New;J. Latham;D. Dunlop;R. Oreffo
人骨髓含有由多能间充质干细胞产生的骨祖细胞。将骨祖细胞植入支架可以产生具有显著机械和生物学潜力的三维活复合材料。本文详细介绍了两个临床病例的实验室和临床结果，其中使用骨髓来源的自体祖细胞增强的影响同种异体移植物治疗不同的股骨近端疾病。自体骨髓植入高度洗净的碎片化同种异体移植物上并进行抑制。同时还采集了受阻移植物的样本进行离体分析。两名患者均获得了简单的临床康复。影像学证实缺损充盈，初始移植物融合良好。组织化学染色和碱性磷酸酶染色结果表明，骨缺损中植入了具有成骨活性的活复合移植物。这些研究表明，骨髓来源的细胞可以粘附在高度洗涤的片状异体移植物上，在嵌塞过程中存活下来，并以成骨细胞表型增殖，从而形成活的复合材料。
Human bone marrow contains bone progenitor cells that arise from multipotent mesenchymal stem cells. Seeding bone progenitor cells onto a scaffold can produce a 3D living composite with significant mechanical and biological potential. This article details laboratory and clinical findings from two clinical cases, where different proximal femoral conditions were treated using impacted allograft augmented with marrow-derived autogenous progenitor cells. Autologous bone marrow was seeded onto highly washed morselized allograft and impacted. Samples of the impacted graft were also taken for ex vivo analysis. Both patients made an uncomplicated clinical recovery. Imaging confirmed defect filling with encouraging initial graft incorporation. Histochemical and alkaline phosphatase staining demonstrated that a live composite graft with osteogenic activity had been introduced into the defects. These studies demonstrate that marrow-derived cells can adhere to highly washed morselized allograft, survive the impaction process and proliferate with an osteoblastic phenotype, thus creating a living composite.