Diverse human extracellular RNAs are widely detected in human plasma.
Diverse human extracellular RNAs are widely detected in human plasma.
复制标题
DOI：
10.1038/ncomms11106
复制
发表时间：
2016-04-26
影响因子：
16.6
通讯作者：
Tanriverdi K
中科院分区：
文献类型：
--
作者：
Freedman JE;Gerstein M;Mick E;Rozowsky J;Levy D;Kitchen R;Das S;Shah R;Danielson K;Beaulieu L;Navarro FC;Wang Y;Galeev TR;Holman A;Kwong RY;Murthy V;Tanriverdi SE;Koupenova-Zamor M;Mikhalev E;Tanriverdi K
人们越来越认识到非蛋白编码基因在发育和疾病中的重要性。尽管我们对microrna了解甚多，但RNA测序的生物信息学分析的局限性阻碍了对人类其他形式的小RNA的广泛评估。通过分析来自40个个体的血浆来源RNA的测序数据，我们鉴定了超过1000种人类细胞外RNA，包括microrna， piwi相互作用RNA （piRNA）和小核仁RNA。在另外2,763个个体中使用靶向定量PCR与逆转录方法，我们鉴定了近500种最丰富的细胞外转录物，包括microrna， pirna和小核核rna。在一个独立的队列中证实了血浆中存在许多非microrna - small- rna。我们提出了全面的数据，以证明广泛和一致的检测不同类别的循环非细胞小rna来自大量人口。细胞外mirna存在于多种体液中。在这里，Freedman等人通过RNA-seq分析了40人的血浆来源RNA，随后在另外2763人中进行了靶向RT-qPCR，并报告了超过1000种细胞外RNA，包括microRNAs、piwi相互作用RNA和小核核RNA。
There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing data from plasma-derived RNA from 40 individuals, here we identified over a thousand human extracellular RNAs including microRNAs, piwi-interacting RNA (piRNA), and small nucleolar RNAs. Using a targeted quantitative PCR with reverse transcription approach in an additional 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population. Extracellular miRNAs are present in a variety of bodily fluids. Here, Freedman et al. analysed plasma-derived RNA by RNA-seq from 40 people followed by targeted RT-qPCR in an additional 2,763 people, and report over 1,000 extracellular RNAs including microRNAs, piwi-interacting RNA and small nucleolar RNAs.