Canine coronavirus-associated puppy mortality without evidence of concurrent canine parvovirus infection
Canine coronavirus-associated puppy mortality without evidence of concurrent canine parvovirus infection
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DOI：
10.1177/104063870501700618
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发表时间：
2005-11-01
影响因子：
1.5
通讯作者：
Han, S
中科院分区：
文献类型：
--
作者：
Evermann, JF;Abbott, JR;Han, S
本报告介绍了2例幼犬死亡与犬冠状病毒（CCV）有关，但没有观察到并发犬细小病毒（CPV-2）疾病的证据。病例1涉及一只7周大的雄性短毛奇瓦瓦州，从宠物店购买后24小时变得昏昏欲睡。在72小时内，小狗开始呕吐，腹泻，并被送往兽医诊所，在那里它被放置在静脉输液。细小病毒Cite（R）试验为阴性。这只小狗在入院后12小时内死亡，并被提交进行诊断检查。大体病理学显示肠炎，提示CPV-2。肠组织病理学显示散在扩张的隐窝，伴坏死细胞碎片和中性粒细胞。淋巴滤泡中度耗竭和坏死。肠道内容物的电子显微镜检查（EM）为冠状病毒阳性，细小病毒阴性。肠道切片的免疫组织化学（IHC）对CCV呈阳性，对CPV-2呈阴性。病例2是一只8周大的雄性西施犬，它被收住兽医诊所，表现出严重胃肠炎伴腹痛的症状。转诊兽医对小狗实施了安乐死，并将整个身体提交进行诊断评估。尸检显示严重的回盲肠肠套叠和小肠节段性坏死性肠炎。肠道内容物的电子显微镜检查结果为冠状病毒阳性，细小病毒阴性。受影响肠道切片的免疫组化结果为CCV阳性，而CPV-2阴性。这些病例强调了当CPV-2疾病已被IHC排除时，在幼犬中进行CCV诊断的重要性。
This report presents 2 cases in which puppy fatalities were associated with canine coronavirus (CCV), but no evidence of concurrent canine parvovirus (CPV-2) disease was observed. Case 1 involved a 7-week-old, male short-haired Chihuahua, which had become lethargic 24 hours after purchase from a pet store. Within 72 hours, the puppy began to vomit, had diarrhea, and was admitted to the veterinary clinic, where it was placed on IV fluids. The parvovirus Cite (R) test was negative. The puppy died within 12 hours of admission and was submitted for diagnostic workup. Gross pathology revealed an enteritis suggestive of CPV-2. Histopathology on intestines showed scattered dilated crypts with necrotic cellular debris and neutrophils. There was moderate depletion and necrosis of lymphoid follicles. Electron microscopy (EM) on intestinal contents was positive for coronavirus and negative for parvovirus. Immunohistochemistry (IHC) on gut sections was positive for CCV and negative for CPV-2. Case 2 was an 8-week-old, male Shih Tzu, which was admitted to the veterinary clinic exhibiting symptoms of severe gastroenteritis with abdominal pain. The referring veterinarian euthanized the puppy, and the entire body was submitted for diagnostic evaluation. Necropsy revealed a severe ileo-cecal intussusception and segmental necrotic enteritis of the small intestine. Electron microscopy of the intestinal contents was positive for coronavirus and negative for parvovirus. Immunohistochemistry on sections of affected gut were positive for CCV and negative for CPV-2. These cases emphasize the importance of pursuing a diagnosis of CCV in young puppies when CPV-2 disease has been ruled out by IHC.