A Novel Electrochemical Approach for Prolonged Measurement of Absolute Levels of Extracellular Dopamine in Brain Slices
A Novel Electrochemical Approach for Prolonged Measurement of Absolute Levels of Extracellular Dopamine in Brain Slices
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DOI：
10.1021/acschemneuro.5b00120
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发表时间：
2015-11-01
影响因子：
5
通讯作者：
Lipski, Janusz
中科院分区：
文献类型：
--
作者：
Burrell, Mark H.;Atcherley, Christopher W.;Lipski, Janusz
强直性多巴胺（DA）水平影响多巴胺能神经元的活动和多巴胺能快速传递的动力学。尽管碳纤维微电极和快速扫描循环伏安法（FSCV）已被广泛用于量化体内和体外刺激诱导的DA释放和摄取，但该技术依赖于背景减法，因此无法提供绝对细胞外浓度的信息。由于背景电流的漂移，它通常也不适合长时间（bbb90秒）的记录。最近报道了一种改进的FSCV方法，称为快速扫描控制吸附伏安法（FSCAV），用于评估溶液和麻醉小鼠大脑中的强直性DA水平。在这里，我们描述了一种新的FSCAV扩展，以研究药物诱导的，缓慢发生的强直性（背景）细胞外DA浓度变化，以及脑切片中阶段性（刺激）DA释放。FSCAV测定吸附动力学和DA浓度变化（最长1.5 h，采样间隔30 s，检测阈值）
Tonic dopamine (DA) levels influence the activity of dopaminergic neurons and the dynamics of fast dopaminergic transmission. Although carbon fiber microelectrodes and fast-scan cyclic voltarnmetry (FSCV) have been extensively used to quantify stimulus-induced release and uptake of DA in vivo and in vitro, this technique relies on background subtraction and thus cannot provide information about absolute extracellular concentrations. It is also generally not suitable for prolonged (>90 s) recordings due to drift of the background current. A recently reported, modified FSCV approach called fast-scan controlled-adsorption voltammetry (FSCAV) has been used to assess tonic DA levels in solution and in the anesthetized mouse brain. Here we describe a novel extension of FSCAV to investigate pharmacologically induced, slowly occurring changes in tonic (background) extracellular DA concentration, and phasic (stimulated) DA release in brain slices. FSCAV was used to measure adsorption dynamics and changes in DA concentration (for up to 1.5 h, sampling interval 30 s, detection threshold