A NOVEL CELL-SURFACE MOLECULE ON EARLY B-LINEAGE CELLS
A NOVEL CELL-SURFACE MOLECULE ON EARLY B-LINEAGE CELLS
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DOI：
10.1038/321616a0
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发表时间：
1986-06-05
期刊：
影响因子：
64.8
通讯作者：
CAZENAVE, PA
中科院分区：
文献类型：
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作者：
COOPER, MD;MULVANEY, D;CAZENAVE, PA
B细胞及其抗体分泌的后代是造血干细胞(HSC)可能进入的多种分化途径之一1，2。代表HSC和B细胞之间的中间阶段的细胞在哺乳动物的造血组织中已被鉴定3，4并在过去的十年中得到了深入的研究。这群早期的B细胞被称为Pre-B，其特征是细胞增殖和免疫球蛋白基因重排的有序级联5-7，这是一系列事件的组合，导致产生克隆性多样的B细胞，然后迁移到周围淋巴组织。哪些因素决定了前B细胞的多克隆生长，免疫球蛋白基因重排是如何调节的，以及前B细胞经历了非生产性的免疫球蛋白基因重排会发生什么，这还有待确定。如果能够准确地识别和分离早期的B系细胞，这些问题可能会更容易解决。在这里，我们描述了一种细胞表面糖蛋白，它是由小鼠造血组织中的前B细胞和新形成的B细胞选择性表达的。这种分子是由相对分子质量(MR)为140,000的二硫键连接链形成的同源二聚体，由一种名为BP-1的鼠单抗鉴定。
B cells and their antibody-secreting progeny represent one of several differentiation pathways that haematopoietic stem cells (HSC) may enter1,2. Cells representing intermediate stages between HSC and B cells have been identified in mammalian haematopoietic tissues3,4and studied intensively over the past decade. This population of early B-lineage cells, termed pre-B, is characterized by cellular proliferation and an orderly cascade of immunoglobulin gene rearrangements5–7, a combination of events leading to the generation of clonally diverse B cells which then migrate to peripheral lymphoid tissues. It remains to be determined what elements determine the polyclonal growth of pre-B cells, how immunoglobulin gene rearrangements are regulated, and what happens to pre-B cells undergoing ‘non-productive’ immunoglobulin gene rearrangements. These issues could be resolved more easily if early B-lineage cells could be identified precisely and isolated. Here, we describe a cell surface glycoprotein that is selectively expressed by pre-B and newly formed B cells in murine haematopoietic tissues. The molecule, a homodimer formed by disulphide-linked chains of relative molecular mass (Mr) 140,000, is identified by a mouse monoclonal alloantibody called BP-1.